Overlapping deletions define novel embryonic lethal loci in the mouse t complex

Hanna H.J. Chao, Sarah E. Mentzer, John C. Schimenti, Yun You

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The t complex region of mouse chromosome 17 contains genetic information critical for embryonic development. To Identify and map loci required for normal embryogenesis, a set of overlapping deletions (D17Aus9df10J, D17Aus9df12J, and D17Aus9df13J) surrounding the D17Aus9 locus and one encompassing the T locus, Del(17)7J, were bred in various combinations and the consequences of nullizygosity in overlapping regions were examined. The results indicated that there are at least two functional units within 1 cM of D17Ausg. /17J1 is a peri-implantation lethal mutation within the region deleted in D17Aus9df13J, whereas /17J2 is a lateracting lethal defined by the region of overlap between Del(17)T7J and D17Aus9df12J. Del(17)T7J/D17Aus9df12J embryos die around 10.5 dpc. The development of the mutant embryos is characterized by lack of axial rotation, an abnormal notochord structure, and a ballooning pericardium. These studies demonstrate the value of overlapping deletion complexes, as opposed to individual deletion complexes, for the identification, mapping, and analysis of genes required for embryonic development.

Original languageEnglish (US)
Pages (from-to)133-142
Number of pages10
JournalGenesis
Volume35
Issue number2
DOIs
StatePublished - Feb 1 2003

Keywords

  • Chromosome deletions
  • Embryonic lethal
  • Mouse
  • T complex

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