Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications

Daniel Senfter; Erdogan Pekcan Erkan; Erdener Özer; Gerhard Jungwirth; Sibylle Madlener; Marcel Kool; Thomas Ströbel; Nurten Saydam; Okay Saydam

doi:10.1002/pbc.26670

Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications

Daniel Senfter, Erdogan Pekcan Erkan, Erdener Özer, Gerhard Jungwirth, Sibylle Madlener, Marcel Kool, Thomas Ströbel, Nurten Saydam, Okay Saydam

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Background: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs. Procedure: We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. Results: Our data showed that most of the pre-RC components are significantly overexpressed in MB. Among all pre-RC mRNAs, MCM10 showed the highest level of expression (∼500- to 1,000-fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells. Conclusions: Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.

Original language	English (US)
Article number	e26670
Journal	Pediatric Blood and Cancer
Volume	64
Issue number	12
DOIs	https://doi.org/10.1002/pbc.26670
State	Published - Dec 2017
Externally published	Yes

Bibliographical note

Funding Information:
The authors declare that there is no conflict of interest. Support for this work was provided, in part, by Melodie Privatstiftung (N.S. and O.S.), EU-FP7-PEOPLE-2011-CIG (O.S.), and the Children?s Cancer Research Institute, St. Anna Kinderkrebsforschung-0017 (O.S.). We thank Begum Alural for her help with the artwork.

Publisher Copyright:
© 2017 Wiley Periodicals, Inc.

Keywords

MCM10
medulloblastoma
minichromosome maintenance protein
prereplication complex

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10.1002/pbc.26670

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title = "Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications",

abstract = "Background: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs. Procedure: We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. Results: Our data showed that most of the pre-RC components are significantly overexpressed in MB. Among all pre-RC mRNAs, MCM10 showed the highest level of expression (∼500- to 1,000-fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells. Conclusions: Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.",

keywords = "MCM10, medulloblastoma, minichromosome maintenance protein, prereplication complex",

author = "Daniel Senfter and Erkan, {Erdogan Pekcan} and Erdener {\"O}zer and Gerhard Jungwirth and Sibylle Madlener and Marcel Kool and Thomas Str{\"o}bel and Nurten Saydam and Okay Saydam",

note = "Funding Information: The authors declare that there is no conflict of interest. Support for this work was provided, in part, by Melodie Privatstiftung (N.S. and O.S.), EU-FP7-PEOPLE-2011-CIG (O.S.), and the Children?s Cancer Research Institute, St. Anna Kinderkrebsforschung-0017 (O.S.). We thank Begum Alural for her help with the artwork. Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",

year = "2017",

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doi = "10.1002/pbc.26670",

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AU - Senfter, Daniel

AU - Erkan, Erdogan Pekcan

AU - Özer, Erdener

AU - Jungwirth, Gerhard

AU - Madlener, Sibylle

AU - Kool, Marcel

AU - Ströbel, Thomas

AU - Saydam, Nurten

AU - Saydam, Okay

N1 - Funding Information: The authors declare that there is no conflict of interest. Support for this work was provided, in part, by Melodie Privatstiftung (N.S. and O.S.), EU-FP7-PEOPLE-2011-CIG (O.S.), and the Children?s Cancer Research Institute, St. Anna Kinderkrebsforschung-0017 (O.S.). We thank Begum Alural for her help with the artwork. Publisher Copyright: © 2017 Wiley Periodicals, Inc.

PY - 2017/12

Y1 - 2017/12

N2 - Background: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs. Procedure: We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. Results: Our data showed that most of the pre-RC components are significantly overexpressed in MB. Among all pre-RC mRNAs, MCM10 showed the highest level of expression (∼500- to 1,000-fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells. Conclusions: Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.

AB - Background: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs. Procedure: We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. Results: Our data showed that most of the pre-RC components are significantly overexpressed in MB. Among all pre-RC mRNAs, MCM10 showed the highest level of expression (∼500- to 1,000-fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells. Conclusions: Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.

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KW - minichromosome maintenance protein

KW - prereplication complex

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Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications

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