Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications

Daniel Senfter, Erdogan Pekcan Erkan, Erdener Özer, Gerhard Jungwirth, Sibylle Madlener, Marcel Kool, Thomas Ströbel, Nurten Saydam, Okay Saydam

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Background: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs. Procedure: We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. Results: Our data showed that most of the pre-RC components are significantly overexpressed in MB. Among all pre-RC mRNAs, MCM10 showed the highest level of expression (∼500- to 1,000-fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells. Conclusions: Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.

Original languageEnglish (US)
Article numbere26670
JournalPediatric Blood and Cancer
Volume64
Issue number12
DOIs
StatePublished - Dec 2017

Bibliographical note

Funding Information:
The authors declare that there is no conflict of interest. Support for this work was provided, in part, by Melodie Privatstiftung (N.S. and O.S.), EU-FP7-PEOPLE-2011-CIG (O.S.), and the Children?s Cancer Research Institute, St. Anna Kinderkrebsforschung-0017 (O.S.). We thank Begum Alural for her help with the artwork.

Keywords

  • MCM10
  • medulloblastoma
  • minichromosome maintenance protein
  • prereplication complex

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