Background: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs. Procedure: We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines. Results: Our data showed that most of the pre-RC components are significantly overexpressed in MB. Among all pre-RC mRNAs, MCM10 showed the highest level of expression (∼500- to 1,000-fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells. Conclusions: Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.
Bibliographical noteFunding Information:
The authors declare that there is no conflict of interest. Support for this work was provided, in part, by Melodie Privatstiftung (N.S. and O.S.), EU-FP7-PEOPLE-2011-CIG (O.S.), and the Children?s Cancer Research Institute, St. Anna Kinderkrebsforschung-0017 (O.S.). We thank Begum Alural for her help with the artwork.
- minichromosome maintenance protein
- prereplication complex