Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: High-level production and some properties of functional expressed lipase B

E. Catoni, C. Schmidt-Dannert, S. Brocca, R. D. Schmid

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Lipase A and B from Geotrichum candidum CMICC 335426 were functionally expressed and secreted in Pichia pastoris. Lipase B exhibits a unique substrate specificity for long-chain cis-Δ9 unsaturated triacylglycerols. Thus, using different cultivation conditions, the production of recombinant lipase B was optimised, yielding 200 000 Units per liter culture. After overexpression lipase B was purified by diafiltration (372 U/mg). The investigated enzyme properties were comparable to those reported for the native enzyme.

Original languageEnglish (US)
Pages (from-to)689-695
Number of pages7
JournalBiotechnology Techniques
Volume11
Issue number9
DOIs
StatePublished - Jan 1 1997

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