Overexpression of IbP5CR enhances salt tolerance in transgenic sweetpotato

Degao Liu; Shaozhen He; Hong Zhai; Lianjun Wang; Yao Zhao; Bing Wang; Ruijie Li; Qingchang Liu

doi:10.1007/s11240-013-0415-y

Overexpression of IbP5CR enhances salt tolerance in transgenic sweetpotato

Degao Liu, Shaozhen He, Hong Zhai, Lianjun Wang, Yao Zhao, Bing Wang, Ruijie Li, Qingchang Liu

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Pyrroline-5-carboxylate reductase (P5CR) lies at the converging point of the glutamate and ornithine pathways and is the last and critical enzyme in proline biosynthesis. In the present study, a P5CR gene, named IbP5CR, was isolated from salt-tolerant sweetpotato line ND98. Expression of IbP5CR was up-regulated in sweetpotato under salt stress. The IbP5CR-overexpressing sweetpotato (cv. Kokei No. 14) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content and superoxide dismutase and photosynthetic activities were significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. H₂O₂ was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbP5CR up-regulated pyrroline-5-carboxylate synthase gene and down-regulated proline dehydrogenase and P5C dehydrogenase genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS) scavenging genes was found in the transgenic plants under salt stress. These findings suggest that overexpression of IbP5CR increases proline accumulation, which enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system. This study indicates that IbP5CR gene has the potential to be used for improving salt tolerance of plants.

Original language	English (US)
Pages (from-to)	1-16
Number of pages	16
Journal	Plant Cell, Tissue and Organ Culture
Volume	117
Issue number	1
DOIs	https://doi.org/10.1007/s11240-013-0415-y
State	Published - Apr 2014
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments We thank Dr. Michael Portereiko, Ceres, Inc. USA, and Dr. Daniel Q. Tong, University of Maryland, USA, for English improvement. We also thank Prof. Wang T, State Key Laboratory of Agrobiotechnology, Beijing, China, for providing Strain EHA 105. This work was supported by the National Natural Science Foundation of China (31071468), China Agriculture Research System (CARS-11, Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008).

Keywords

IbP5CR
Proline
ROS scavenging system
Salt tolerance
Sweetpotato

Publisher link

10.1007/s11240-013-0415-y

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title = "Overexpression of IbP5CR enhances salt tolerance in transgenic sweetpotato",

abstract = "Pyrroline-5-carboxylate reductase (P5CR) lies at the converging point of the glutamate and ornithine pathways and is the last and critical enzyme in proline biosynthesis. In the present study, a P5CR gene, named IbP5CR, was isolated from salt-tolerant sweetpotato line ND98. Expression of IbP5CR was up-regulated in sweetpotato under salt stress. The IbP5CR-overexpressing sweetpotato (cv. Kokei No. 14) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content and superoxide dismutase and photosynthetic activities were significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbP5CR up-regulated pyrroline-5-carboxylate synthase gene and down-regulated proline dehydrogenase and P5C dehydrogenase genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS) scavenging genes was found in the transgenic plants under salt stress. These findings suggest that overexpression of IbP5CR increases proline accumulation, which enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system. This study indicates that IbP5CR gene has the potential to be used for improving salt tolerance of plants.",

keywords = "IbP5CR, Proline, ROS scavenging system, Salt tolerance, Sweetpotato",

author = "Degao Liu and Shaozhen He and Hong Zhai and Lianjun Wang and Yao Zhao and Bing Wang and Ruijie Li and Qingchang Liu",

note = "Funding Information: Acknowledgments We thank Dr. Michael Portereiko, Ceres, Inc. USA, and Dr. Daniel Q. Tong, University of Maryland, USA, for English improvement. We also thank Prof. Wang T, State Key Laboratory of Agrobiotechnology, Beijing, China, for providing Strain EHA 105. This work was supported by the National Natural Science Foundation of China (31071468), China Agriculture Research System (CARS-11, Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008).",

year = "2014",

month = apr,

doi = "10.1007/s11240-013-0415-y",

language = "English (US)",

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T1 - Overexpression of IbP5CR enhances salt tolerance in transgenic sweetpotato

AU - Liu, Degao

AU - He, Shaozhen

AU - Zhai, Hong

AU - Wang, Lianjun

AU - Zhao, Yao

AU - Wang, Bing

AU - Li, Ruijie

AU - Liu, Qingchang

N1 - Funding Information: Acknowledgments We thank Dr. Michael Portereiko, Ceres, Inc. USA, and Dr. Daniel Q. Tong, University of Maryland, USA, for English improvement. We also thank Prof. Wang T, State Key Laboratory of Agrobiotechnology, Beijing, China, for providing Strain EHA 105. This work was supported by the National Natural Science Foundation of China (31071468), China Agriculture Research System (CARS-11, Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008).

PY - 2014/4

Y1 - 2014/4

N2 - Pyrroline-5-carboxylate reductase (P5CR) lies at the converging point of the glutamate and ornithine pathways and is the last and critical enzyme in proline biosynthesis. In the present study, a P5CR gene, named IbP5CR, was isolated from salt-tolerant sweetpotato line ND98. Expression of IbP5CR was up-regulated in sweetpotato under salt stress. The IbP5CR-overexpressing sweetpotato (cv. Kokei No. 14) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content and superoxide dismutase and photosynthetic activities were significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbP5CR up-regulated pyrroline-5-carboxylate synthase gene and down-regulated proline dehydrogenase and P5C dehydrogenase genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS) scavenging genes was found in the transgenic plants under salt stress. These findings suggest that overexpression of IbP5CR increases proline accumulation, which enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system. This study indicates that IbP5CR gene has the potential to be used for improving salt tolerance of plants.

AB - Pyrroline-5-carboxylate reductase (P5CR) lies at the converging point of the glutamate and ornithine pathways and is the last and critical enzyme in proline biosynthesis. In the present study, a P5CR gene, named IbP5CR, was isolated from salt-tolerant sweetpotato line ND98. Expression of IbP5CR was up-regulated in sweetpotato under salt stress. The IbP5CR-overexpressing sweetpotato (cv. Kokei No. 14) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content and superoxide dismutase and photosynthetic activities were significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbP5CR up-regulated pyrroline-5-carboxylate synthase gene and down-regulated proline dehydrogenase and P5C dehydrogenase genes under salt stress. The systemic up-regulation of reactive oxygen species (ROS) scavenging genes was found in the transgenic plants under salt stress. These findings suggest that overexpression of IbP5CR increases proline accumulation, which enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and activating ROS scavenging system. This study indicates that IbP5CR gene has the potential to be used for improving salt tolerance of plants.

KW - IbP5CR

KW - Proline

KW - ROS scavenging system

KW - Salt tolerance

KW - Sweetpotato

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DO - 10.1007/s11240-013-0415-y

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SN - 0167-6857

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Overexpression of IbP5CR enhances salt tolerance in transgenic sweetpotato

Abstract

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