Over-expression of GL-7-ACA acylase gene in E.coli

Chen Lu Lin; Qiang Li; Ya Fei Liu; Bo Wang

Over-expression of GL-7-ACA acylase gene in E.coli

Chen Lu Lin, Qiang Li, Ya Fei Liu, Bo Wang

Research output: Contribution to journal › Article › peer-review

Abstract

The constitutive expression of glutaryl-7-amidocephalosporanic acid (GL-7-ACA) acylase was realized. Based on it, a new plasmid pGEMKT-HPfACY with optimized RBS was constructed and transformed into E. coli JM105.After culturing in LB media, this recombinant strain reached an activity of 0.44 U/mL. This activity was twice of former one while the activity of the strain JM105/pGEMKT-HPRfACY reached as high as 2.98 U/mL using corn-steep as culture media. The acylase activity of recombinant strain hit 6.37 U/mL, using a strategy of controlling pH 7.5 by adding nutrient such as glycerin.

Original language	English (US)
Pages (from-to)	140-143
Number of pages	4
Journal	Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering
Volume	8
Issue number	1
State	Published - Jan 2008
Externally published	Yes

Keywords

7-aminocephalosporanic acid
Constitutive expression
Glutaryl-7-amidocephalosporanic acid acylase

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abstract = "The constitutive expression of glutaryl-7-amidocephalosporanic acid (GL-7-ACA) acylase was realized. Based on it, a new plasmid pGEMKT-HPfACY with optimized RBS was constructed and transformed into E. coli JM105.After culturing in LB media, this recombinant strain reached an activity of 0.44 U/mL. This activity was twice of former one while the activity of the strain JM105/pGEMKT-HPRfACY reached as high as 2.98 U/mL using corn-steep as culture media. The acylase activity of recombinant strain hit 6.37 U/mL, using a strategy of controlling pH 7.5 by adding nutrient such as glycerin.",

keywords = "7-aminocephalosporanic acid, Constitutive expression, Glutaryl-7-amidocephalosporanic acid acylase",

author = "Lin, {Chen Lu} and Qiang Li and Liu, {Ya Fei} and Bo Wang",

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journal = "Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering",

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T1 - Over-expression of GL-7-ACA acylase gene in E.coli

AU - Lin, Chen Lu

AU - Li, Qiang

AU - Liu, Ya Fei

AU - Wang, Bo

PY - 2008/1

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N2 - The constitutive expression of glutaryl-7-amidocephalosporanic acid (GL-7-ACA) acylase was realized. Based on it, a new plasmid pGEMKT-HPfACY with optimized RBS was constructed and transformed into E. coli JM105.After culturing in LB media, this recombinant strain reached an activity of 0.44 U/mL. This activity was twice of former one while the activity of the strain JM105/pGEMKT-HPRfACY reached as high as 2.98 U/mL using corn-steep as culture media. The acylase activity of recombinant strain hit 6.37 U/mL, using a strategy of controlling pH 7.5 by adding nutrient such as glycerin.

AB - The constitutive expression of glutaryl-7-amidocephalosporanic acid (GL-7-ACA) acylase was realized. Based on it, a new plasmid pGEMKT-HPfACY with optimized RBS was constructed and transformed into E. coli JM105.After culturing in LB media, this recombinant strain reached an activity of 0.44 U/mL. This activity was twice of former one while the activity of the strain JM105/pGEMKT-HPRfACY reached as high as 2.98 U/mL using corn-steep as culture media. The acylase activity of recombinant strain hit 6.37 U/mL, using a strategy of controlling pH 7.5 by adding nutrient such as glycerin.

KW - 7-aminocephalosporanic acid

KW - Constitutive expression

KW - Glutaryl-7-amidocephalosporanic acid acylase

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Over-expression of GL-7-ACA acylase gene in E.coli

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