Ovarian carcinoma ascites spheroids adhere to extracellular matrix components and mesothelial cell monolayers

Kathryn M. Burleson, Rachael C. Casey, Keith M Skubitz, Stephan E. Pambuccian, Theodore R. Oegema, Amy P Skubitz

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Objective. Ovarian carcinoma cells form multicellular aggregates, or spheroids, in the peritoneal cavity of patients with advanced disease. The current paradigm that ascites spheroids are non-adhesive leaves their contribution to ovarian carcinoma dissemination undefined. Here, spheroids obtained from ovarian carcinoma patients' ascites were characterized for their ability to adhere to molecules encountered in the peritoneal cavity, with the goal of establishing their potential to contribute to ovarian cancer spread. Methods. Spheroids were recovered from the ascites fluid of 11 patients with stage III or stage IV ovarian carcinoma. Adhesion assays to extracellular matrix (ECM) proteins and human mesothelial cell monolayers were performed for each of the ascites spheroid samples. Subsequently, inhibition assays were performed to identify the cell receptors involved. Results. Most ascites samples adhered moderately to fibronectin and type I collagen, with reduced adhesion to type IV collagen and laminin. Monoclonal antibodies against the β1 integrin subunit partially inhibited this adhesion. Ascites spheroids also adhered to hyaluronan. Additionally, spheroids adhered to live, but not fixed, human mesothelial cell monolayers, and this adhesion was partially mediated by β1 integrins. Conclusions. The cellular content of the ascites fluid has often been considered non-adhesive, but our findings are the first to suggest that patient-derived ascites spheroids can adhere to mesothelial extracellular matrix via β1 integrins, indicating that spheroids should not be ignored in the dissemination of ovarian cancer.

Original languageEnglish (US)
Pages (from-to)170-181
Number of pages12
JournalGynecologic oncology
Volume93
Issue number1
DOIs
StatePublished - Apr 1 2004

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Cellular Structures
Ascites
Extracellular Matrix
Carcinoma
Integrins
Peritoneal Cavity
Ovarian Neoplasms
Collagen Type IV
Extracellular Matrix Proteins
Laminin
Hyaluronic Acid
Collagen Type I
Fibronectins
Cell Adhesion
Monoclonal Antibodies

Keywords

  • Cell adhesion molecules
  • Extracellular matrix
  • Integrins
  • Ovarian carcinoma
  • Spheroids

Cite this

Ovarian carcinoma ascites spheroids adhere to extracellular matrix components and mesothelial cell monolayers. / Burleson, Kathryn M.; Casey, Rachael C.; Skubitz, Keith M; Pambuccian, Stephan E.; Oegema, Theodore R.; Skubitz, Amy P.

In: Gynecologic oncology, Vol. 93, No. 1, 01.04.2004, p. 170-181.

Research output: Contribution to journalArticle

Burleson, Kathryn M. ; Casey, Rachael C. ; Skubitz, Keith M ; Pambuccian, Stephan E. ; Oegema, Theodore R. ; Skubitz, Amy P. / Ovarian carcinoma ascites spheroids adhere to extracellular matrix components and mesothelial cell monolayers. In: Gynecologic oncology. 2004 ; Vol. 93, No. 1. pp. 170-181.
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