Otx5 regulates genes that show circadian expression in the zebrafish pineal complex

Joshua T. Gamse, Yu Chi Shen, Christine Thisse, Bernard Thisse, Pamela A. Raymond, Marnie E. Halpern, Jennifer O. Liang

Research output: Contribution to journalArticlepeer-review

138 Scopus citations


The photoneuroendocrine system translates environmental light conditions into the circadian production of endocrine and neuroendocrine signals. Central to this process is the pineal organ, which has a conserved role in the cyclical synthesis and release of melatonin to influence sleep patterns and seasonal reproduction. In lower vertebrates, the pineal organ contains photoreceptors whose activity entrains an endogenous circadian clock and regulates transcription in pinealocytes. In mammals, pineal function is influenced by retinal photoreceptors that project to the suprachiasmatic nucleus - the site of the endogenous circadian clock. A multisynaptic pathway then relays information about circadian rhythmicity and photoperiod to the pineal organ. The gene cone rod homeobox (crx), a member of the orthodenticle homeobox (otx) family, is thought to regulate pineal circadian activity. In the mouse, targeted inactivation of Crx causes a reduction in pineal gene expression and attenuated entrainment to light/dark cycles. Here we show that crx and otx5 orthologs are expressed in both the pineal organ and the asymmetrically positioned parapineal of larval zebrafish. Circadian gene expression is unaffected by a reduction in Crx expression but is inhibited specifically by depletion of Otx5. Our results indicate that Otx5 rather than Crx regulates genes that show circadian expression in the zebrafish pineal complex.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalNature Genetics
Issue number1
StatePublished - Jan 2002

Bibliographical note

Funding Information:
We thank M. Macurak, J. Prowell and L. Hantsoo for technical help. This work was supported by an American Cancer Society postdoctoral fellowship (J.T.G.), the Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, Hôpital Universitaire de Strasbourg, Association pour la Recherche sur le Cancer, Ligue Nationale Contre le Cancer (C.T., B.T.), NIH grants (C.T., B.T., P.A.R.) and an NRSA fellowship (J.O.L.).


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