TY - JOUR
T1 - Osteogenic protein-1 induces bone for formation in the presence of bacterial infection in a rat intramuscular osteoinduction model
AU - Chen, Xinqian
AU - Kidder, Louis S.
AU - Schmidt, Andrew H
AU - Lew, William D.
PY - 2004/8
Y1 - 2004/8
N2 - Objective: Evaluate the ability of osteogenic protein-1 to induce formation of de novo bone in the presence of bacterial infection and metal in an intramuscular osteoinduction model in the rat. Design: Prospective experimental design with assessment time points of up to 4 weeks. Setting: Intramuscular pocket surgically created along each side of the spine. Animals: One-hundred-twenty adult male Sprague-Dawley rats. Interventions: Each intramuscular pocket received 0, 10, or 25 μg of osteogenic protein-1 combined with a lyophilized collagen carrier, and 0 or 5 × 105 colony-forming units of Staphylococcus aureus. Pockets in 48 animals received a metal implant. Animals were killed at 1, 2, 3, or 4 weeks. Main Outcome Measurements: High-resolution radiographs of resulting nodules of bone/soft tissue were digitized, and areas of newly formed bone were quantified using an image analysis workstation. The nodules were decalcified for histology, and calcium content of the decalcifying solution was quantified by flame atomic absorption spectrophotometry. Results: There were minimal levels of calcium and area of new bone formation in nodules from pockets containing collagen carrier without osteogenic protein-1, for both infection and noninfection conditions. Calcium content and area of newly formed bone were significantly greater: 1) in infected pockets with osteogenic protein-1, compared to infected pockets without osteogenic protein-1; and 2) in noninfected pockets with osteogenic protein-1, compared to infected pockets with osteogenic protein-1. The presence of metal did not have a significant effect. Conclusion: Osteogenic protein-1 maintained its osteoinductive capability in a contaminated intramuscular pocket in the rat, albeit at a lower level than without infection. This finding supports further study using a more clinically realistic model.
AB - Objective: Evaluate the ability of osteogenic protein-1 to induce formation of de novo bone in the presence of bacterial infection and metal in an intramuscular osteoinduction model in the rat. Design: Prospective experimental design with assessment time points of up to 4 weeks. Setting: Intramuscular pocket surgically created along each side of the spine. Animals: One-hundred-twenty adult male Sprague-Dawley rats. Interventions: Each intramuscular pocket received 0, 10, or 25 μg of osteogenic protein-1 combined with a lyophilized collagen carrier, and 0 or 5 × 105 colony-forming units of Staphylococcus aureus. Pockets in 48 animals received a metal implant. Animals were killed at 1, 2, 3, or 4 weeks. Main Outcome Measurements: High-resolution radiographs of resulting nodules of bone/soft tissue were digitized, and areas of newly formed bone were quantified using an image analysis workstation. The nodules were decalcified for histology, and calcium content of the decalcifying solution was quantified by flame atomic absorption spectrophotometry. Results: There were minimal levels of calcium and area of new bone formation in nodules from pockets containing collagen carrier without osteogenic protein-1, for both infection and noninfection conditions. Calcium content and area of newly formed bone were significantly greater: 1) in infected pockets with osteogenic protein-1, compared to infected pockets without osteogenic protein-1; and 2) in noninfected pockets with osteogenic protein-1, compared to infected pockets with osteogenic protein-1. The presence of metal did not have a significant effect. Conclusion: Osteogenic protein-1 maintained its osteoinductive capability in a contaminated intramuscular pocket in the rat, albeit at a lower level than without infection. This finding supports further study using a more clinically realistic model.
KW - Atomic absorption spectrophotometry
KW - Calcium ion content
KW - Infection
KW - Intramuscular osteoinduction model
KW - Osteogenic protein-1
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U2 - 10.1097/00005131-200408000-00008
DO - 10.1097/00005131-200408000-00008
M3 - Article
C2 - 15289690
AN - SCOPUS:4444272194
SN - 0890-5339
VL - 18
SP - 436
EP - 442
JO - Journal of orthopaedic trauma
JF - Journal of orthopaedic trauma
IS - 7
ER -