Osteogenic and adipogenic differentiation potential of an immortalized fibroblast-like cell line derived from porcine peripheral blood

Xiuqing Wang, Daphne Moutsoglou

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Primary fibroblast-like cells isolated from the peripheral blood of a healthy pig were immortalized by transduction of cells with a replication-defective retrovirus vector expressing the E6/E7 proteins of human papillomavirus type 16 (pLXSN-16E6E7). The immortalized cells grow rapidly in cell culture and exhibit a distinct cell surface phenotype that was positive for CD90, CD44, collagen I, and vimentin and negative for CD14 and MHC II. Additionally, these immortalized blood derivedfibroblast-like cells had the potential to differentiate into osteoblasts and adipocytes in vitro as evidenced by the deposition of calcium, increased alkaline phosphatase activity, upregulated osteogenic and adipogenic marker gene expression, and accumulation of fat droplets in cells when osteogenic (dexamethasone, ascorbic acid, and β-glycerophosphate) or adipogenic supplements (dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin, and insulin) were added to the culture. Overall, the results suggest that the immortalized blood-derived fibroblast-like cells exhibit some of the features of mesenchymal precursor cells, which may have implications in tissue repair and remodeling process.

Original languageEnglish (US)
Pages (from-to)584-591
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume45
Issue number10
DOIs
StatePublished - Oct 2009

Bibliographical note

Funding Information:
Acknowledgements We thank Dr. Hongmei Li for the technical help in oil red O staining. We also thank Dr. Jeff Clapper and Mr. Dean Peters for providing us with pig blood. This work was partly supported by the South Dakota Agriculture Experiment Station and by NSF/EPSCoR #EPS-0091948 and by the State of South Dakota.

Keywords

  • Adipogenic differentiation
  • Fibroblast-like cells
  • Immortalization
  • Mesenchymal precursor cells
  • Osteogenic differentiation
  • Porcine

Fingerprint

Dive into the research topics of 'Osteogenic and adipogenic differentiation potential of an immortalized fibroblast-like cell line derived from porcine peripheral blood'. Together they form a unique fingerprint.

Cite this