Osteoblastäre differenzierung von humanen adulten mesenchymalen stammzellen durch transgenes BMP-2 in abwesenheit von dexamethason

Translated title of the contribution: Osteoblastic differentiation of human adult mesenchymal stem cells after Ad-BMP-2 in the absence of dexamethasone

Hannjörg Koch, J. A. Jadlowiec, J. D. Whalen, P. Robbins, C. Lattermann, F. H. Fu, H. R. Merk, J. O. Hollinger

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Aim: Mesenchymal stem cells (MSC) of various species appear to require different cues to differentiate towards the osteoblastic lineage. For MSC of human origin, recombinant hBMP-2 is reported to be not sufficient but dexamethasone seems to be essential. The aim of this study was to analyse changes in genotype and phenotype of hMSC after adenoviral transfer of the BMP-2 gene in the absence of dexamethasone. Methods: We employed hMSC and analysed changes in expression of the Runx2, Osterix and type I collagen gene by quantitative PCR after adenoviral transfer of the human BMP-2 gene in the absence of dexamethasone. As a phenotypic marker alkaline phosphatase activity was assessed. ANOVA and post hoc statistical analyses were used to determine differences among data (p < 0.05). Results: Transfer of the hBMP-2 gene and consecutive production of transgenic BMP-2 up-regulated bone marker gene expression and increased alkaline phosphatase activity and thus promoted an enhanced lineage progression to the osteoblast phenotype without the addition of dexamethasone. Conclusion: These findings are noteworthy in the light of a possible superiority of endogenous transgenic proteins compared to exogenous recombinant proteins.

Translated title of the contributionOsteoblastic differentiation of human adult mesenchymal stem cells after Ad-BMP-2 in the absence of dexamethasone
Original languageGerman
Pages (from-to)684-690
Number of pages7
JournalZeitschrift fur Orthopadie und Ihre Grenzgebiete
Volume143
Issue number6
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Keywords

  • Adenoviral transduction
  • BMP-2
  • Human mesenchymal stem cells
  • Osteoblastic differentiation
  • Quantitative real-time PCR

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