Orphanin FQ/nociceptin-immunoreactive nerve fibers parallel those containing endogenous opioids in rat spinal cord

M. Riedl, S. Shuster, L. Vulchanova, J. Wang, H. Loh, R. Elde

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

Antisera were developed that specifically recognize orphanin FQ/nociceptin, the 17 amino acid peptide reported to be the endogenous ligand for the orphan opioid receptor. Immunocytochemical localizations in rat spinal cord demonstrated that orphanin FQ/nociceptin-immunoreactivity (-ir) was abundant in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal, areas that also exhibit prominent enkephalin- and dynorphin-ir. Orphanin FQ/nociceptin-ir was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. Thus, the distribution of orphanin FQ/nociceptin-ir appeared in neuronal circuits that parallel those containing enkephalin- and dynorphin-ir, with only modest co-existence of these peptides.

Original languageEnglish (US)
Pages (from-to)1369-1372
Number of pages4
JournalNeuroreport
Volume7
Issue number8
DOIs
StatePublished - Jan 1 1996

Keywords

  • Dynorphin
  • Enkephalin
  • Immunoreactivity
  • Orphan opioid receptor
  • Rat
  • Spinal cord

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