TY - JOUR
T1 - Orphan opioid receptor oligonucleotides inhibit HIV-1 expression in human brain cells
AU - Chao, Chun C.
AU - Gekker, Genya
AU - Sheng, Wen
AU - Hu, Shuxian
AU - Loh, Horace H
AU - Peterson, Phillip K.
PY - 1998
Y1 - 1998
N2 - We found that orphan opioid receptor (OR) mRNA was constitutively expressed in human fetal microglia, astrocytes, and cerebral cortical neurons. An OR antisense oligonucleotide markedly inhibited HIV-1 expression in enriched microglial cell and the mixed glial/neuronal cell cultures but not in the chronically infected promonocytic U1 cells. The sense oligonucleotide also inhibited HIV-1 expression in brain cell cultures. These findings indicate that while human brain cells constitutively express mRNA for OR, antisense oligonucleotides to OR non-specifically inhibit acute HIV- 1 infection.
AB - We found that orphan opioid receptor (OR) mRNA was constitutively expressed in human fetal microglia, astrocytes, and cerebral cortical neurons. An OR antisense oligonucleotide markedly inhibited HIV-1 expression in enriched microglial cell and the mixed glial/neuronal cell cultures but not in the chronically infected promonocytic U1 cells. The sense oligonucleotide also inhibited HIV-1 expression in brain cell cultures. These findings indicate that while human brain cells constitutively express mRNA for OR, antisense oligonucleotides to OR non-specifically inhibit acute HIV- 1 infection.
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U2 - 10.1007/978-1-4615-5347-2_10
DO - 10.1007/978-1-4615-5347-2_10
M3 - Article
C2 - 9666260
AN - SCOPUS:0031857016
SN - 0065-2598
VL - 437
SP - 83
EP - 90
JO - Advances in experimental medicine and biology
JF - Advances in experimental medicine and biology
ER -
