Ornithine decarboxylase overexpression leads to increased epithelial tumor invasiveness

Mary K. Smith, Mary A. Goral, Jane H. Wright, Lynn M. Matrisian, Rebecca J. Morris, Andres J.P. Klein-Szanto, Susan K. Gilmour

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Ornithine decarboxylase (ODC) overexpression cooperates with genetic lesions such as an activated c-ras(IIa) to enhance epithelial tumorigenesis. To assess the invasiveness of ODC-overexpressing cells, two noninvasive epidermal cell lines, nontumorigenic BK-1 cells, and the papilloma-derived cell line SP-1 were infected with a replication-defective retrovirus that overexpresses ODC, inoculated into deepithelialized rat tracheas, and transplanted into athymic nude mice. After 5 weeks, ODC-overexpressing BK-1 cells remained localized on the luminal surface of the tracheal xenotransplants, whereas the ODC-overexpressing SP-1 cells were extremely invasive, with the whole tracheal wall penetrated. This invasiveness of ODC- overexpressing SP-1 cells was accompanied by elevated proteinase expression, including increased urokinase plasminogen activator activity in ODC- overexpressing cells and elevated stromelysin-1 mRNA expression in the stromal cells of invaded tracheal transplants.

Original languageEnglish (US)
Pages (from-to)2104-2108
Number of pages5
JournalCancer Research
Volume57
Issue number11
StatePublished - Jun 1 1997
Externally publishedYes

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    Smith, M. K., Goral, M. A., Wright, J. H., Matrisian, L. M., Morris, R. J., Klein-Szanto, A. J. P., & Gilmour, S. K. (1997). Ornithine decarboxylase overexpression leads to increased epithelial tumor invasiveness. Cancer Research, 57(11), 2104-2108.