Ornithine decarboxylase overexpression leads to increased epithelial tumor invasiveness

Mary K. Smith; Mary A. Goral; Jane H. Wright; Lynn M. Matrisian; Rebecca J. Morris; Andres J.P. Klein-Szanto; Susan K. Gilmour

Ornithine decarboxylase overexpression leads to increased epithelial tumor invasiveness

Mary K. Smith, Mary A. Goral, Jane H. Wright, Lynn M. Matrisian, Rebecca J. Morris, Andres J.P. Klein-Szanto, Susan K. Gilmour

Research output: Contribution to journal › Article › peer-review

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Abstract

Ornithine decarboxylase (ODC) overexpression cooperates with genetic lesions such as an activated c-ras(IIa) to enhance epithelial tumorigenesis. To assess the invasiveness of ODC-overexpressing cells, two noninvasive epidermal cell lines, nontumorigenic BK-1 cells, and the papilloma-derived cell line SP-1 were infected with a replication-defective retrovirus that overexpresses ODC, inoculated into deepithelialized rat tracheas, and transplanted into athymic nude mice. After 5 weeks, ODC-overexpressing BK-1 cells remained localized on the luminal surface of the tracheal xenotransplants, whereas the ODC-overexpressing SP-1 cells were extremely invasive, with the whole tracheal wall penetrated. This invasiveness of ODC- overexpressing SP-1 cells was accompanied by elevated proteinase expression, including increased urokinase plasminogen activator activity in ODC- overexpressing cells and elevated stromelysin-1 mRNA expression in the stromal cells of invaded tracheal transplants.

Original language	English (US)
Pages (from-to)	2104-2108
Number of pages	5
Journal	Cancer Research
Volume	57
Issue number	11
State	Published - Jun 1 1997
Externally published	Yes

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title = "Ornithine decarboxylase overexpression leads to increased epithelial tumor invasiveness",

abstract = "Ornithine decarboxylase (ODC) overexpression cooperates with genetic lesions such as an activated c-ras(IIa) to enhance epithelial tumorigenesis. To assess the invasiveness of ODC-overexpressing cells, two noninvasive epidermal cell lines, nontumorigenic BK-1 cells, and the papilloma-derived cell line SP-1 were infected with a replication-defective retrovirus that overexpresses ODC, inoculated into deepithelialized rat tracheas, and transplanted into athymic nude mice. After 5 weeks, ODC-overexpressing BK-1 cells remained localized on the luminal surface of the tracheal xenotransplants, whereas the ODC-overexpressing SP-1 cells were extremely invasive, with the whole tracheal wall penetrated. This invasiveness of ODC- overexpressing SP-1 cells was accompanied by elevated proteinase expression, including increased urokinase plasminogen activator activity in ODC- overexpressing cells and elevated stromelysin-1 mRNA expression in the stromal cells of invaded tracheal transplants.",

author = "Smith, {Mary K.} and Goral, {Mary A.} and Wright, {Jane H.} and Matrisian, {Lynn M.} and Morris, {Rebecca J.} and Klein-Szanto, {Andres J.P.} and Gilmour, {Susan K.}",

year = "1997",

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language = "English (US)",

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T1 - Ornithine decarboxylase overexpression leads to increased epithelial tumor invasiveness

AU - Smith, Mary K.

AU - Goral, Mary A.

AU - Wright, Jane H.

AU - Matrisian, Lynn M.

AU - Morris, Rebecca J.

AU - Klein-Szanto, Andres J.P.

AU - Gilmour, Susan K.

PY - 1997/6/1

Y1 - 1997/6/1

N2 - Ornithine decarboxylase (ODC) overexpression cooperates with genetic lesions such as an activated c-ras(IIa) to enhance epithelial tumorigenesis. To assess the invasiveness of ODC-overexpressing cells, two noninvasive epidermal cell lines, nontumorigenic BK-1 cells, and the papilloma-derived cell line SP-1 were infected with a replication-defective retrovirus that overexpresses ODC, inoculated into deepithelialized rat tracheas, and transplanted into athymic nude mice. After 5 weeks, ODC-overexpressing BK-1 cells remained localized on the luminal surface of the tracheal xenotransplants, whereas the ODC-overexpressing SP-1 cells were extremely invasive, with the whole tracheal wall penetrated. This invasiveness of ODC- overexpressing SP-1 cells was accompanied by elevated proteinase expression, including increased urokinase plasminogen activator activity in ODC- overexpressing cells and elevated stromelysin-1 mRNA expression in the stromal cells of invaded tracheal transplants.

AB - Ornithine decarboxylase (ODC) overexpression cooperates with genetic lesions such as an activated c-ras(IIa) to enhance epithelial tumorigenesis. To assess the invasiveness of ODC-overexpressing cells, two noninvasive epidermal cell lines, nontumorigenic BK-1 cells, and the papilloma-derived cell line SP-1 were infected with a replication-defective retrovirus that overexpresses ODC, inoculated into deepithelialized rat tracheas, and transplanted into athymic nude mice. After 5 weeks, ODC-overexpressing BK-1 cells remained localized on the luminal surface of the tracheal xenotransplants, whereas the ODC-overexpressing SP-1 cells were extremely invasive, with the whole tracheal wall penetrated. This invasiveness of ODC- overexpressing SP-1 cells was accompanied by elevated proteinase expression, including increased urokinase plasminogen activator activity in ODC- overexpressing cells and elevated stromelysin-1 mRNA expression in the stromal cells of invaded tracheal transplants.

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AN - SCOPUS:0031001040

SN - 0008-5472

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JO - Cancer Research

JF - Cancer Research

IS - 11

ER -

Ornithine decarboxylase overexpression leads to increased epithelial tumor invasiveness

Abstract

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