To evaluate risk factors and to approach methods of reduction of the carcinogenic potential of cigarette smoke, the transfer rate of N-nitrosonornicotine in a popular U.S. blended cigarette into mainstream smoke was quantitatively determined. The mean transfer rate was 11.3%; thus ≈46% of the tobacco-specific carcinogen In the smoke came from the tobacco, and the remainder was synthesized during smoking.
Bibliographical noteFunding Information:
ABBREVIATIONS USED: NNN = N'-nitrosonornicotine; GLC = gas liquid chromatography; HPLC = high-pressure liquid chromatography. 1 Received August 4, 1976; accepted November 29, 1976. 2 Supported by Public Health Service contract NOI-CP55666 from the Division of Cancer Cause and Prevention, National Cancer Institute (NCI). 3 No. LII of "Chemical Studies on Tobacco Smoke." 4 Division of Environmental Carcinogenesis, Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, N.Y. 10595. 5 Recipient of Public Health Service Career Development Award 1- K04-CAOOI24 from the NCI. 6 We thank W. Z. Maddox and M. R. Guerin, Analytical Chemistry Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, for supplying an "Automatic Syringe Applicator for Cigarettes."