Organization of the rat Tage4 gene and herpesvirus entry activity of the encoded protein

Béatrice Baury, Robert J. Geraghty, Damien Masson, Patrick Lustenberger, Patricia G. Spear, Marc G. Denis

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The Tage4 gene (Tumor-Associated Glycoprotein E4) is a member of the immunoglobulin superfamily overexpressed in rat colon tumors and Min mouse intestinal adenomas. The Tage4 cDNA presents ∼60% identity with the human CD155, a member of the immunoglobulin superfamily coding for a transmembrane protein capable of serving as an entry receptor for poliovirus, porcine pseudorabies virus and bovine herpesvirus 1. We determined the structure of the Tage4 gene. This gene covers ∼15 kb and is composed of eight exons and seven introns. We also isolated ∼2 kb of the 5′ flanking region of the Tage4 gene and demonstrated the existence of closely clustered transcription start sites. No splicing variant was identified by RT-PCR indicating that the Tage4 gene is transcribed as a unique mRNA. Finally, the protein encoded by the Tage4 gene was tested for ability to mediate entry of several viruses. These structural and functional features of the rat Tage4 gene were compared to those of the human CD155 gene. The results indicated that the Tage4 gene is probably orthologous to the gene for CD155.

Original languageEnglish (US)
Pages (from-to)185-194
Number of pages10
JournalGene
Volume265
Issue number1-2
DOIs
StatePublished - Mar 7 2001
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grants from the Ligue Départementale (44) contre le Cancer (M.G.D.) and from the National Institutes of Health, R37 AI36293 (P.G.S.). B.B. is a recipient of a fellowship from the Ligue Départementale (44) contre le Cancer. R.J.G. was supported by National Research Service Award F32 AI09471. We wish to thank Carl Pavel and Vincent Racaniello for the results on poliovirus susceptibility and for critical reading of the manuscript.

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

  • CD155
  • Cancer cells
  • Immunoglobulin superfamily
  • Overexpression

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