Organization of ganglioside GM1 in phosphatidylcholine bilayers

T. E. Thompson; M. Allietta; R. E. Brown; M. L. Johnson; T. W. Tillack

doi:10.1016/0005-2736(85)90024-0

Organization of ganglioside G_M1 in phosphatidylcholine bilayers

T. E. Thompson, M. Allietta, R. E. Brown, M. L. Johnson, T. W. Tillack

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

Molecules of the ganglioside G_M1 are randomly distributed in liquid-crystalline 1-palmitoyl-2-oleoyl phosphatidylcholine bilayers. This conclusion is based on a freeze-etch electron microscopic study using ferritin-conjugated cholera toxin and cholera toxin alone as ganglioside labels. The average number of G_M1 molecules under a label is calculated by a novel method from the dependence of the fraction of bilayer area covered by the label on the mole fraction of G_M1 in the bilayer.

Original language	English (US)
Pages (from-to)	229-237
Number of pages	9
Journal	BBA - Biomembranes
Volume	817
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(85)90024-0
State	Published - Jul 25 1985
Externally published	Yes

Bibliographical note

Funding Information:
We wish to thank Dr. John Heuser of Washington University for preparing samples with his liquid-helium freezing device. We also wish to acknowledge the advice and helpful criticisms of Dr. IstvMa Sughr. This study was supported by U.S. Public Health Service grants GM-23573 and GM-26234.

Keywords

Cholera toxin
Electron microscopy
Freeze-etch
Ganglioside distribution
Phospholipid bilayer

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Publisher link

10.1016/0005-2736(85)90024-0

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Cite this

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title = "Organization of ganglioside GM1 in phosphatidylcholine bilayers",

abstract = "Molecules of the ganglioside GM1 are randomly distributed in liquid-crystalline 1-palmitoyl-2-oleoyl phosphatidylcholine bilayers. This conclusion is based on a freeze-etch electron microscopic study using ferritin-conjugated cholera toxin and cholera toxin alone as ganglioside labels. The average number of GM1 molecules under a label is calculated by a novel method from the dependence of the fraction of bilayer area covered by the label on the mole fraction of GM1 in the bilayer.",

keywords = "Cholera toxin, Electron microscopy, Freeze-etch, Ganglioside distribution, Phospholipid bilayer",

author = "Thompson, {T. E.} and M. Allietta and Brown, {R. E.} and Johnson, {M. L.} and Tillack, {T. W.}",

note = "Funding Information: We wish to thank Dr. John Heuser of Washington University for preparing samples with his liquid-helium freezing device. We also wish to acknowledge the advice and helpful criticisms of Dr. IstvMa Sughr. This study was supported by U.S. Public Health Service grants GM-23573 and GM-26234.",

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doi = "10.1016/0005-2736(85)90024-0",

language = "English (US)",

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T1 - Organization of ganglioside GM1 in phosphatidylcholine bilayers

AU - Thompson, T. E.

AU - Allietta, M.

AU - Brown, R. E.

AU - Johnson, M. L.

AU - Tillack, T. W.

N1 - Funding Information: We wish to thank Dr. John Heuser of Washington University for preparing samples with his liquid-helium freezing device. We also wish to acknowledge the advice and helpful criticisms of Dr. IstvMa Sughr. This study was supported by U.S. Public Health Service grants GM-23573 and GM-26234.

PY - 1985/7/25

Y1 - 1985/7/25

N2 - Molecules of the ganglioside GM1 are randomly distributed in liquid-crystalline 1-palmitoyl-2-oleoyl phosphatidylcholine bilayers. This conclusion is based on a freeze-etch electron microscopic study using ferritin-conjugated cholera toxin and cholera toxin alone as ganglioside labels. The average number of GM1 molecules under a label is calculated by a novel method from the dependence of the fraction of bilayer area covered by the label on the mole fraction of GM1 in the bilayer.

AB - Molecules of the ganglioside GM1 are randomly distributed in liquid-crystalline 1-palmitoyl-2-oleoyl phosphatidylcholine bilayers. This conclusion is based on a freeze-etch electron microscopic study using ferritin-conjugated cholera toxin and cholera toxin alone as ganglioside labels. The average number of GM1 molecules under a label is calculated by a novel method from the dependence of the fraction of bilayer area covered by the label on the mole fraction of GM1 in the bilayer.

KW - Cholera toxin

KW - Electron microscopy

KW - Freeze-etch

KW - Ganglioside distribution

KW - Phospholipid bilayer

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