Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response

Jagdeep S. Sandhu, Sergei F. Krasnyanski, Leslie L. Domier, Schuyler S. Korban, Mark D. Osadjan, Dennis E. Buetow

Research output: Contribution to journalArticle

129 Scopus citations

Abstract

Respiratory syncytial virus (RSV) is one of the most important pathogens of infancy and early childhood. Here a fruit-based edible subunit vaccine against RSV was developed by expressing the RSV fusion (F) protein gene in transgenic tomato plants. The F-gene was expressed in ripening tomato fruit under the control of the fruit-specific E8 promoter. Oral immunization of mice with ripe transgenic tomato fruits led to the induction of both serum and mucosal RSV-F specific antibodies. The ratio of immunoglobulin subclasses produced in response to immunization suggested that a type 1 T-helper cell immune response was preferentially induced. Serum antibodies showed an increased titer when the immunized mice were exposed to inactivated RSV antigen.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalTransgenic Research
Volume9
Issue number2
DOIs
StatePublished - Aug 21 2000

Keywords

  • Edible vaccine
  • Immunoglobulins G and A
  • Respiratory syncytial virus
  • Transgenic tomato

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