Oral bacteria in plaque and invading buccal cells of young orthodontic patients

Nancy M. Leung, Ruoqiong Chen, Joel D Rudney

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Introduction: In this longitudinal study, patients with fixed orthodontic appliances served as models to determine whether Actinobacillus actinomycetemcomitans, Tannerella forsythia, and total streptococci increased after treatment, and whether treatment affected bacterial invasion into the adjacent buccal epithelial cells (BEC). Methods: Supragingival plaque, subgingival plaque, and BEC were collected from 27 patients before and at least 4 weeks after placement of orthodontic fixed appliances. Total sample DNA was determined, and bacteria were assayed by quantitative polymerase chain reaction. The BEC were also examined by confocal microscopy after fluorescent in-situ hybridization to visually detect the presence of each species bacteria in BEC. Results: Total DNA in supragingival and subgingival plaque increased after appliance placement (P = .005). There was also a significant increase in supragingival streptococci (P = .0002). By confocal microscopy, a trend toward fewer buccal cells recovered was found after appliance placement, and there was a significant increase in the percentage of buccal cells containing A. actinomycetemcomitans (P = .0058). Conclusions: Appliance placement appeared to increase buccal cell susceptibility to A. actinomycetemcomitans invasion. This might be due to physical trauma or to leaching of metals from the appliances.

Original languageEnglish (US)
JournalAmerican Journal of Orthodontics and Dentofacial Orthopedics
Volume130
Issue number6
DOIs
StatePublished - Dec 1 2006

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Cheek
Orthodontics
Bacteria
Aggregatibacter actinomycetemcomitans
Epithelial Cells
Orthodontic Appliances
Streptococcus
Confocal Microscopy
DNA
Fluorescence In Situ Hybridization
Longitudinal Studies
Metals
Polymerase Chain Reaction
Wounds and Injuries
Therapeutics

Cite this

Oral bacteria in plaque and invading buccal cells of young orthodontic patients. / Leung, Nancy M.; Chen, Ruoqiong; Rudney, Joel D.

In: American Journal of Orthodontics and Dentofacial Orthopedics, Vol. 130, No. 6, 01.12.2006.

Research output: Contribution to journalArticle

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