Plackett-Burman design was used to optimize various media compositions of carbon and nitrogen sources along with other nutrient parameters influencing the production of compactin under submerge fermentation. Initially eight different carbon sources were tested individually for compactin production. Among them glucose containing medium had produce maximum compactin. Secondly eight different nitrogen sources were tested, in which the medium containing urea and soybean meal had produce maximum compactin. The above carbon and nitrogen source was chosen for further optimization, eight nutrients such as (glucose, glycerol, soya bean meal, KH2PO4, urea, PEG, CaCl3.H2O and MgSO4.7H2O) were chosen for Plackett-Burman design with a 12 experimental runs. Among 8 factors studied by Plackett-Burman design, three factors had influenced the compactin production. The MgSO4.7H2O, KH2PO4 and glycerol medium constituents were found to be effective in the production of compactin by Penicillium citrinum. An improved compactin yield of 232.11 mg/L was obtained with optimized medium composition, which is higher production than previously obtained results.
|Original language||English (US)|
|Number of pages||8|
|Journal||International Journal of ChemTech Research|
|State||Published - May 28 2013|
- Penicillium citrinum
- Plackett-burman design and submerge fermentation