Optimization of a microfluidic device for diffusion-based extraction of DMSO from a cell suspension

K. K. Fleming Glass, E. K. Longmire, A. Hubel

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26 Scopus citations

Abstract

This study considers the use of a two stream microfluidic device for extraction of dimethyl sulfoxide (DMSO) from a cryopreserved cell suspension. The DMSO diffuses from a cell suspension stream into a neighboring wash stream flowing in parallel. The model of Fleming et al. [K.K. Fleming, E.K. Longmire, A. Hubel, Numerical characterization of diffusion-based extraction in a cell-laden flow through a microfluidic channel, Journal of Biomechanical Engineering 129 (2007) 703-711] is employed to determine and discuss optimal geometry and operating conditions for a case requiring removal of 95% DMSO from suspension streams with volumetric flow rates up to 2.5 ml/min. The effects of Peclet number, flow rate fraction, and cell volume fraction are analyzed, and expansion of the analysis to other applications is discussed.

Original languageEnglish (US)
Pages (from-to)5749-5757
Number of pages9
JournalInternational Journal of Heat and Mass Transfer
Volume51
Issue number23-24
DOIs
StatePublished - Nov 2008

Keywords

  • Cell suspension
  • Channel flow
  • Diffusion
  • Optimization

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