Optical mapping of electrical heterogeneities in the heart during global ischemia

Arvydas Matiukas, Arkady M. Pertsov, P. Kothari, A. Cram, Elena G. Tolkacheva

Research output: Chapter in Book/Report/Conference proceedingConference contribution

13 Citations (Scopus)

Abstract

Real-time optical registration of electrical activity in the heart allows the study of arrhythmogenic mechanisms, in particular due to global ischemia. It is known that global ischemia increases electrical heterogeneity in the heart. However, inter-ventricular differences between the right (RV) and left ventricle (LV) during ischemia and their relationship to arrhythmogenesis remains poorly understood. We used high resolution optical mapping (di-4-ANEPPS, excitation at 532nm, emission at 640±50 nm) of Langendorff-perfused rabbit hearts to quantify inter-ventricular heterogeneity in the heart during periodic pacing and ventricular fibrillation. Two fast CCD cameras were used to record electrical activity from the RV and LV during control, global ischemia (20 min), and reperfusion. Hearts were paced at progressively reduced (from 300 ms to 100 ms) basic cycle lengths and ventricular fibrillation was induced by burst pacing and recorded before the global ischemia, and after the reperfusion. The action potential durations (APD), maximum slopes of APD restitution curves (Smax), and mean dominant frequency (DF) of ventricular fibrillation were measured for both LV and RV surfaces. No APD heterogeneity was observed in control hearts. Global ischemia induced inter-ventricular heterogeneity in APDs (RV: 109±21 ms, LV: 89±23 ms; p<0.01) that was abolished upon reperfusion. However, Smax was uniformly decreased in both RV (control: 0.94±0.25, ischemia: 0.36±0.12; p<0.01) and LV (control: 0.99±0.24, ischemia: 0.43±0.21; p<0.01) and did not recover upon reperfusion. In addition, the DF of ventricular fibrillation during reperfusion decreased significantly in RV (from 8.6±1.3 Hz to 6.2±1.1 Hz; p<0.05) but remained the same in LV (9.0±0.8 Hz vs 8.5±1.0 Hz). Thus, our results demonstrate that global ischemia induces interventricular heterogeneity in APD during periodic pacing. Although this effect was abolished upon reperfusion, Smax did not recover, indicating the presence of residual changes in electrical properties of the heart. Therefore, reperfusion reveals the presence of inter-ventricular heterogeneities in the dynamics of ventricular fibrillation.

Original languageEnglish (US)
Title of host publicationProceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
Subtitle of host publicationEngineering the Future of Biomedicine, EMBC 2009
PublisherIEEE Computer Society
Pages6321-6324
Number of pages4
ISBN (Print)9781424432967
DOIs
StatePublished - 2009
Event31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009 - Minneapolis, MN, United States
Duration: Sep 2 2009Sep 6 2009

Publication series

NameProceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009

Other

Other31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
CountryUnited States
CityMinneapolis, MN
Period9/2/099/6/09

Fingerprint

Ischemia
Reperfusion
Heart Ventricles
Ventricular Fibrillation
Action Potentials
pamidronate
CCD cameras
Electric properties
Rabbits

Cite this

Matiukas, A., Pertsov, A. M., Kothari, P., Cram, A., & Tolkacheva, E. G. (2009). Optical mapping of electrical heterogeneities in the heart during global ischemia. In Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009 (pp. 6321-6324). [5333176] (Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009). IEEE Computer Society. https://doi.org/10.1109/IEMBS.2009.5333176

Optical mapping of electrical heterogeneities in the heart during global ischemia. / Matiukas, Arvydas; Pertsov, Arkady M.; Kothari, P.; Cram, A.; Tolkacheva, Elena G.

Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009. IEEE Computer Society, 2009. p. 6321-6324 5333176 (Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Matiukas, A, Pertsov, AM, Kothari, P, Cram, A & Tolkacheva, EG 2009, Optical mapping of electrical heterogeneities in the heart during global ischemia. in Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009., 5333176, Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, IEEE Computer Society, pp. 6321-6324, 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, Minneapolis, MN, United States, 9/2/09. https://doi.org/10.1109/IEMBS.2009.5333176
Matiukas A, Pertsov AM, Kothari P, Cram A, Tolkacheva EG. Optical mapping of electrical heterogeneities in the heart during global ischemia. In Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009. IEEE Computer Society. 2009. p. 6321-6324. 5333176. (Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009). https://doi.org/10.1109/IEMBS.2009.5333176
Matiukas, Arvydas ; Pertsov, Arkady M. ; Kothari, P. ; Cram, A. ; Tolkacheva, Elena G. / Optical mapping of electrical heterogeneities in the heart during global ischemia. Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009: Engineering the Future of Biomedicine, EMBC 2009. IEEE Computer Society, 2009. pp. 6321-6324 (Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009).
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abstract = "Real-time optical registration of electrical activity in the heart allows the study of arrhythmogenic mechanisms, in particular due to global ischemia. It is known that global ischemia increases electrical heterogeneity in the heart. However, inter-ventricular differences between the right (RV) and left ventricle (LV) during ischemia and their relationship to arrhythmogenesis remains poorly understood. We used high resolution optical mapping (di-4-ANEPPS, excitation at 532nm, emission at 640±50 nm) of Langendorff-perfused rabbit hearts to quantify inter-ventricular heterogeneity in the heart during periodic pacing and ventricular fibrillation. Two fast CCD cameras were used to record electrical activity from the RV and LV during control, global ischemia (20 min), and reperfusion. Hearts were paced at progressively reduced (from 300 ms to 100 ms) basic cycle lengths and ventricular fibrillation was induced by burst pacing and recorded before the global ischemia, and after the reperfusion. The action potential durations (APD), maximum slopes of APD restitution curves (Smax), and mean dominant frequency (DF) of ventricular fibrillation were measured for both LV and RV surfaces. No APD heterogeneity was observed in control hearts. Global ischemia induced inter-ventricular heterogeneity in APDs (RV: 109±21 ms, LV: 89±23 ms; p<0.01) that was abolished upon reperfusion. However, Smax was uniformly decreased in both RV (control: 0.94±0.25, ischemia: 0.36±0.12; p<0.01) and LV (control: 0.99±0.24, ischemia: 0.43±0.21; p<0.01) and did not recover upon reperfusion. In addition, the DF of ventricular fibrillation during reperfusion decreased significantly in RV (from 8.6±1.3 Hz to 6.2±1.1 Hz; p<0.05) but remained the same in LV (9.0±0.8 Hz vs 8.5±1.0 Hz). Thus, our results demonstrate that global ischemia induces interventricular heterogeneity in APD during periodic pacing. Although this effect was abolished upon reperfusion, Smax did not recover, indicating the presence of residual changes in electrical properties of the heart. Therefore, reperfusion reveals the presence of inter-ventricular heterogeneities in the dynamics of ventricular fibrillation.",
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