Optic nerve as a source of activated retinal microglia post-injury

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Using mice expressing green fluorescent protein (GFP) from a transgenic CD11c promoter we found that a controlled optic nerve crush (ONC) injury attracted GFPhi retinal myeloid cells to the dying retinal ganglion cells and their axons. However, the origin of these retinal myeloid cells was uncertain. In this study we use transgenic mice in conjunction with ONC, partial and full optic nerve transection (ONT), and parabiosis to determine the origin of injury induced retinal myeloid cells. Analysis of parabiotic mice and fate mapping showed that responding retinal myeloid cells were not derived from circulating macrophages and that GFPhi myeloid cells could be derived from GFPlo microglia. Comparison of optic nerve to retina following an ONC showed a much greater concentration of GFPhi cells and GFPlo microglia in the optic nerve. Optic nerve injury also induced Ki67+ cells in the optic nerve but not in the retina. Comparison of the retinal myeloid cell response after full versus partial ONT revealed fewer GFPhi cells and GFPlo microglia in the retina following a full ONT despite it being a more severe injury, suggesting that full transection of the optic nerve can block the migration of responding myeloid cells to the retina. Our results suggest that the optic nerve can be a reservoir for activated microglia and other retinal myeloid cells in the retina following optic nerve injury.

Original languageEnglish (US)
Number of pages1
JournalActa Neuropathologica Communications
Volume6
Issue number1
DOIs
StatePublished - Jul 23 2018

Fingerprint

Microglia
Myeloid Cells
Optic Nerve Injuries
Optic Nerve
Wounds and Injuries
Retina
Nerve Crush
Parabiosis
Retinal Ganglion Cells
Nerve Block
Green Fluorescent Proteins
Transgenic Mice
Axons
Macrophages

Keywords

  • Injury response
  • Microglia
  • Migration
  • Optic nerve
  • Origin
  • Retina

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

Cite this

Optic nerve as a source of activated retinal microglia post-injury. / Heuss, Neal D; Pierson, Mark J.; Roehrich, Heidi; Mc Pherson, Scott W; Gram, Andrea L.; Li, Ling; Gregerson, Dale S.

In: Acta Neuropathologica Communications, Vol. 6, No. 1, 23.07.2018.

Research output: Contribution to journalArticle

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