A new mid-infrared beamline (IRENI) extracting 320 hor. × 25 vert. mrads 2 of radiation providing wide-field illumination for a Bruker Hyperion 3000 IR microscope equipped with an infrared focal plane array (FPA) detector has recently been commissioned at the Synchrotron Radiation Center (SRC) in Stoughton, WI. The swath of radiation from the SRC is extracted as 12 beams and recombined into a 3 × 4 bundle of beams that is refocused onto the sample plane of the infrared microscope illuminating a 40 μm × 60 μm sample area. With this new facility chemical images with high diffraction-limited resolution, for wavelengths across the mid-IR concurrently, can be obtained in minutes. Applications of biomedical, biological and cultural heritage interest show the broad applicability of this new facility. The examples presented here include images of fixed single cells, time dependent in vivo measurements of a single cell and layered organic/inorganic materials.
Bibliographical noteFunding Information:
This work is based upon experiments performed at the Synchrotron Radiation Center on the IRENI beamline. The SRC is funded by the NSF under award number DMR-0537588, and the IRENI beamline was funded by the NSF-MRI program under award number MRI-0619759, and the work was funded by the NSF-CHE under award number 1112433.
- Cultural heritage
- Focal plane array
- Mie scattering
- Mouse embryonic stem cells
- Synchrotron-based infrared imaging