A rat brain cDNA library was screened with probes constructed from portions of the cDNA (OBCAM) encoding the opioid-binding cell adhesion molecule (OBCAM). Three clones of interest were isolated and sequenced. The largest clone, DUZ1, had a putative open reading frame (ORF) essentially identical to OBCAM in its C-terminal 318 amino acids (aa), but differing in its N-terminal aa (20 vs. 27 in OBCAM), and in all of its 5′-noncoding regions. The other clones, SG8 and SG13, had a putative ORF essentially identical to that of OBCAM, but differed from each other in a portion of their 5′-noncoding region. This study suggests that there is a family of OBCAM-like genes.
|Original language||English (US)|
|Number of pages||6|
|State||Published - Aug 15 1992|
Bibliographical noteFunding Information:
This work was supported in part by NIDA research grants 00564 (H.H.L.), 05695 (H.H.L.), 01583 (H.H.L.), 02643 (N.M.L.), and Research Scientist Award K05-DA70554 (H.H.L.) and K05-DA00020 (N.M.L.) and the F. Stark endowed Fund in Minnesota Medical Foundation. D.A.L. is currently registered in the graduate program in the Department of Pharmacology, University of California, San Francisco.
- Opioid receptor
- cDNA cloning
- cell adhesion molecules
- immunoglobulin superfamily