Opioid-binding cell adhesion molecule (OBCAM)-related clones from a rat brain cDNA library

David A. Lippman; Nancy M. Lee; Horace H Loh

doi:10.1016/0378-1119(92)90734-7

Opioid-binding cell adhesion molecule (OBCAM)-related clones from a rat brain cDNA library

David A. Lippman, Nancy M. Lee, Horace H Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A rat brain cDNA library was screened with probes constructed from portions of the cDNA (OBCAM) encoding the opioid-binding cell adhesion molecule (OBCAM). Three clones of interest were isolated and sequenced. The largest clone, DUZ1, had a putative open reading frame (ORF) essentially identical to OBCAM in its C-terminal 318 amino acids (aa), but differing in its N-terminal aa (20 vs. 27 in OBCAM), and in all of its 5′-noncoding regions. The other clones, SG8 and SG13, had a putative ORF essentially identical to that of OBCAM, but differed from each other in a portion of their 5′-noncoding region. This study suggests that there is a family of OBCAM-like genes.

Original language	English (US)
Pages (from-to)	249-254
Number of pages	6
Journal	Gene
Volume	117
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(92)90734-7
State	Published - Aug 15 1992

Keywords

Opioid receptor
cDNA cloning
cell adhesion molecules
immunoglobulin superfamily

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title = "Opioid-binding cell adhesion molecule (OBCAM)-related clones from a rat brain cDNA library",

abstract = "A rat brain cDNA library was screened with probes constructed from portions of the cDNA (OBCAM) encoding the opioid-binding cell adhesion molecule (OBCAM). Three clones of interest were isolated and sequenced. The largest clone, DUZ1, had a putative open reading frame (ORF) essentially identical to OBCAM in its C-terminal 318 amino acids (aa), but differing in its N-terminal aa (20 vs. 27 in OBCAM), and in all of its 5′-noncoding regions. The other clones, SG8 and SG13, had a putative ORF essentially identical to that of OBCAM, but differed from each other in a portion of their 5′-noncoding region. This study suggests that there is a family of OBCAM-like genes.",

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AU - Lippman, David A.

AU - Lee, Nancy M.

AU - Loh, Horace H

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KW - Opioid receptor

KW - cDNA cloning

KW - cell adhesion molecules

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