Opioid-binding cell adhesion molecule (OBCAM)-related clones from a rat brain cDNA library

David A. Lippman; Nancy M. Lee; Horace H Loh

doi:10.1016/0378-1119(92)90734-7

Opioid-binding cell adhesion molecule (OBCAM)-related clones from a rat brain cDNA library

David A. Lippman, Nancy M. Lee, Horace H Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

Abstract

A rat brain cDNA library was screened with probes constructed from portions of the cDNA (OBCAM) encoding the opioid-binding cell adhesion molecule (OBCAM). Three clones of interest were isolated and sequenced. The largest clone, DUZ1, had a putative open reading frame (ORF) essentially identical to OBCAM in its C-terminal 318 amino acids (aa), but differing in its N-terminal aa (20 vs. 27 in OBCAM), and in all of its 5′-noncoding regions. The other clones, SG8 and SG13, had a putative ORF essentially identical to that of OBCAM, but differed from each other in a portion of their 5′-noncoding region. This study suggests that there is a family of OBCAM-like genes.

Original language	English (US)
Pages (from-to)	249-254
Number of pages	6
Journal	Gene
Volume	117
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(92)90734-7
State	Published - Aug 15 1992

Bibliographical note

Funding Information:
This work was supported in part by NIDA research grants 00564 (H.H.L.), 05695 (H.H.L.), 01583 (H.H.L.), 02643 (N.M.L.), and Research Scientist Award K05-DA70554 (H.H.L.) and K05-DA00020 (N.M.L.) and the F. Stark endowed Fund in Minnesota Medical Foundation. D.A.L. is currently registered in the graduate program in the Department of Pharmacology, University of California, San Francisco.

Keywords

Opioid receptor
cDNA cloning
cell adhesion molecules
immunoglobulin superfamily

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title = "Opioid-binding cell adhesion molecule (OBCAM)-related clones from a rat brain cDNA library",

abstract = "A rat brain cDNA library was screened with probes constructed from portions of the cDNA (OBCAM) encoding the opioid-binding cell adhesion molecule (OBCAM). Three clones of interest were isolated and sequenced. The largest clone, DUZ1, had a putative open reading frame (ORF) essentially identical to OBCAM in its C-terminal 318 amino acids (aa), but differing in its N-terminal aa (20 vs. 27 in OBCAM), and in all of its 5′-noncoding regions. The other clones, SG8 and SG13, had a putative ORF essentially identical to that of OBCAM, but differed from each other in a portion of their 5′-noncoding region. This study suggests that there is a family of OBCAM-like genes.",

keywords = "Opioid receptor, cDNA cloning, cell adhesion molecules, immunoglobulin superfamily",

author = "Lippman, {David A.} and Lee, {Nancy M.} and Loh, {Horace H}",

note = "Funding Information: This work was supported in part by NIDA research grants 00564 (H.H.L.), 05695 (H.H.L.), 01583 (H.H.L.), 02643 (N.M.L.), and Research Scientist Award K05-DA70554 (H.H.L.) and K05-DA00020 (N.M.L.) and the F. Stark endowed Fund in Minnesota Medical Foundation. D.A.L. is currently registered in the graduate program in the Department of Pharmacology, University of California, San Francisco.",

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doi = "10.1016/0378-1119(92)90734-7",

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AU - Lippman, David A.

AU - Lee, Nancy M.

AU - Loh, Horace H

N1 - Funding Information: This work was supported in part by NIDA research grants 00564 (H.H.L.), 05695 (H.H.L.), 01583 (H.H.L.), 02643 (N.M.L.), and Research Scientist Award K05-DA70554 (H.H.L.) and K05-DA00020 (N.M.L.) and the F. Stark endowed Fund in Minnesota Medical Foundation. D.A.L. is currently registered in the graduate program in the Department of Pharmacology, University of California, San Francisco.

PY - 1992/8/15

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N2 - A rat brain cDNA library was screened with probes constructed from portions of the cDNA (OBCAM) encoding the opioid-binding cell adhesion molecule (OBCAM). Three clones of interest were isolated and sequenced. The largest clone, DUZ1, had a putative open reading frame (ORF) essentially identical to OBCAM in its C-terminal 318 amino acids (aa), but differing in its N-terminal aa (20 vs. 27 in OBCAM), and in all of its 5′-noncoding regions. The other clones, SG8 and SG13, had a putative ORF essentially identical to that of OBCAM, but differed from each other in a portion of their 5′-noncoding region. This study suggests that there is a family of OBCAM-like genes.

AB - A rat brain cDNA library was screened with probes constructed from portions of the cDNA (OBCAM) encoding the opioid-binding cell adhesion molecule (OBCAM). Three clones of interest were isolated and sequenced. The largest clone, DUZ1, had a putative open reading frame (ORF) essentially identical to OBCAM in its C-terminal 318 amino acids (aa), but differing in its N-terminal aa (20 vs. 27 in OBCAM), and in all of its 5′-noncoding regions. The other clones, SG8 and SG13, had a putative ORF essentially identical to that of OBCAM, but differed from each other in a portion of their 5′-noncoding region. This study suggests that there is a family of OBCAM-like genes.

KW - Opioid receptor

KW - cDNA cloning

KW - cell adhesion molecules

KW - immunoglobulin superfamily

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Opioid-binding cell adhesion molecule (OBCAM)-related clones from a rat brain cDNA library

Abstract

Bibliographical note

Keywords

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