TY - JOUR
T1 - Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla
AU - Lee, Joseph
AU - Hu, Qingsong
AU - Nakamura, Yasuhiro
AU - Wang, Xiaohong
AU - Zhang, Xiaoliang
AU - Zhu, Xiaohong
AU - Chen, Wei
AU - Yang, Qinglin
AU - Zhang, Jianyi
PY - 2006/12
Y1 - 2006/12
N2 - Purpose: To develop a rapid, robust, and accurate method for assessing myocardial energetics in mice and demonstrate its applicability to mouse models of acquired and genetic heart disease. Materials and Methods: We combined surface coil localization (10-mm diameter, tunable between 1H and 31P, using adiabatic half-passage radiofrequency pulses) and surgery (electrocautery removal of anterior chest wall) to create an open-chest method for acquiring in vivo 31P nuclear magnetic resonance (NMR) cardiac spectra from mice at 4.7T within 12 minutes. Normal BALB/c mice, BALB/c with myocardial infarction (MI), cardiomyocyte-restricted peroxisome proliferator-activated receptor-delta knockout (KO) (CR-PPARd-/-) and control loxP-flanked Ppard (Ppardflox/flox) mice were examined. Results: The mean phosphocreatine (PCr)/adenosine triphosphate (ATP) ratios in control BALB/c mice, BALB/c MI mice, Ppardflox/flox mice, and PPAR-δ KO mice were 2.13 ± 0.09 (N = 11), 1.35 ± 0.07 (N = 9, P < 0.001 vs. BALB/c control), 1.92 ± 0.09 (N = 5), and 1.31 ± 0.12 (N = 5, P < 0.005 vs. Ppardflox/flox control), respectively. The significant depression of myocardial PCr/ATP we observed in these genetic/acquired models of heart disease was in accord with previous data from analogous large animal models. No NMR signal contamination from chamber blood or adjacent skeletal muscle was identified. Conclusion: This new technique provides cardiac 31P spectra suitable for accurate quantitative analysis in a relatively short acquisition time, is suitable for terminal studies of mouse myocardial energy metabolism, and could be installed in virtually any NMR laboratory to study myocardial energetics in numerous mouse models of human heart disease.
AB - Purpose: To develop a rapid, robust, and accurate method for assessing myocardial energetics in mice and demonstrate its applicability to mouse models of acquired and genetic heart disease. Materials and Methods: We combined surface coil localization (10-mm diameter, tunable between 1H and 31P, using adiabatic half-passage radiofrequency pulses) and surgery (electrocautery removal of anterior chest wall) to create an open-chest method for acquiring in vivo 31P nuclear magnetic resonance (NMR) cardiac spectra from mice at 4.7T within 12 minutes. Normal BALB/c mice, BALB/c with myocardial infarction (MI), cardiomyocyte-restricted peroxisome proliferator-activated receptor-delta knockout (KO) (CR-PPARd-/-) and control loxP-flanked Ppard (Ppardflox/flox) mice were examined. Results: The mean phosphocreatine (PCr)/adenosine triphosphate (ATP) ratios in control BALB/c mice, BALB/c MI mice, Ppardflox/flox mice, and PPAR-δ KO mice were 2.13 ± 0.09 (N = 11), 1.35 ± 0.07 (N = 9, P < 0.001 vs. BALB/c control), 1.92 ± 0.09 (N = 5), and 1.31 ± 0.12 (N = 5, P < 0.005 vs. Ppardflox/flox control), respectively. The significant depression of myocardial PCr/ATP we observed in these genetic/acquired models of heart disease was in accord with previous data from analogous large animal models. No NMR signal contamination from chamber blood or adjacent skeletal muscle was identified. Conclusion: This new technique provides cardiac 31P spectra suitable for accurate quantitative analysis in a relatively short acquisition time, is suitable for terminal studies of mouse myocardial energy metabolism, and could be installed in virtually any NMR laboratory to study myocardial energetics in numerous mouse models of human heart disease.
KW - Carbon substrate preference
KW - Cardiac NMR
KW - Myocardial energy metabolism
KW - Myocardial infarction
KW - PPARd
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U2 - 10.1002/jmri.20766
DO - 10.1002/jmri.20766
M3 - Article
C2 - 17096395
AN - SCOPUS:33845331405
SN - 1053-1807
VL - 24
SP - 1269
EP - 1276
JO - Journal of Magnetic Resonance Imaging
JF - Journal of Magnetic Resonance Imaging
IS - 6
ER -