One-Step Real-Time Reverse Transcription-PCR for the Detection of Turkey Reoviruses

Sunil K. Mor, Harsha Verma, Aschalew Z. Bekele, Tamer A. Sharafeldin, Robert E. Porter, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

During late 2010 and early 2011, an unusual problem of lameness and swollen hock joints in commercial Turkeys was reported in the upper Midwest, which continues to this day. The disease caused substantial economic losses to Turkey producers. Reovirus was isolated from tendons and joint fluids of lame Turkeys submitted to the Minnesota Veterinary Diagnostic Laboratory. This study was undertaken to develop a TaqMan real-time reverse transcription-PCR (rRT-PCR) assay for the early detection of Turkey reoviruses (both enteric and lameness strains). A primer probe set was designed from the conserved region of the S4 segment of the Turkey reovirus genome. The newly developed rRT-PCR was specific for the detection of Turkey reoviruses. The detection limit of this assay was 10 genome copies per reaction. For the TARV-MN4 strain of Turkey arthritis reovirus, one 50% tissue culture infectious dose was equivalent to 11.6 ± 0.2 genome copies. The highest coefficient of variation for intraexperimental and interexperimental variability was 0.08 and 0.06, respectively, indicating the reproducibility of the assay. This new test should be useful for the detection of Turkey enteric and arthritis reoviruses.

Original languageEnglish (US)
Pages (from-to)404-407
Number of pages4
JournalAvian diseases
Volume58
Issue number3
DOIs
StatePublished - Sep 1 2014

Keywords

  • S4 gene
  • Taqman
  • Turkey reovirus
  • real-time RT-PCR

Fingerprint

Dive into the research topics of 'One-Step Real-Time Reverse Transcription-PCR for the Detection of Turkey Reoviruses'. Together they form a unique fingerprint.

Cite this