On the origins of cyanuric acid hydrolase: Purification, substrates, and prevalence of AtzD from Pseudomonas sp. strain ADP

Isaac Fruchey, Nir Shapir, Michael J. Sadowsky, Lawrence P. Wackett

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Cyanuric acid hydrolase (AtzD) from Pseudomonas sp. strain ADP was purified to homogeneity. Of 22 cyclic amides and triazine compounds tested, only cyanuric acid and N-methylisocyanuric acid were substrates. Other cyclic amidases were found not to hydrolyze cyanuric acid. Ten bacteria that use cyanuric acid as a sole nitrogen source for growth were found to contain either atzD or trzD, but not both genes.

Original languageEnglish (US)
Pages (from-to)3653-3657
Number of pages5
JournalApplied and environmental microbiology
Volume69
Issue number6
DOIs
StatePublished - Jun 1 2003

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