On the geometric characteristics of cell membrane using rotationally oscillating drill (Ros-Drill©)

Jhon Diaz, Mehdi Karzar Jeddi, Nejat Olgac, Tai Hsi Fan, Ali Fuat Ergenc

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

ICSI (intracytoplasmic sperm injection) is a broadly utilized assisted reproductive technology. A number of new versions of the procedure have evolved lately, such as piezo-assisted ICSI technique. An important problem with this technique, however, is that it requires small amounts of mercury to stabilize the pipette tip. A completely different and mercury-free technology, called the "Ros-Drill©" (rotationally oscillating drill) was developed by the group of the authors. It uses microprocessor-controlled rotational oscillations of a spiked micropipette for piercing. One of the key issues is to determine when to start the oscillations. It is based on the cell deformation prior to the membrane piercing. In-situ measurements are needed for this protocol. The contribution of this paper is the utilization of computer vision for these measurements. The triggering logic is correlated to the cell membrane curvature variations along the vision-detected membrane line segment. Such a tool becomes very helpful for automating the Ros-Drill operation.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME Dynamic Systems and Control Conference 2009, DSCC2009
PublisherAmerican Society of Mechanical Engineers(ASME)
Pages1009-1015
Number of pages7
EditionPART B
ISBN (Print)9780791848920
StatePublished - 2010
Externally publishedYes
Event2009 ASME Dynamic Systems and Control Conference, DSCC2009 - Hollywood, CA, United States
Duration: Oct 12 2009Oct 14 2009

Publication series

NameProceedings of the ASME Dynamic Systems and Control Conference 2009, DSCC2009
NumberPART B

Other

Other2009 ASME Dynamic Systems and Control Conference, DSCC2009
Country/TerritoryUnited States
CityHollywood, CA
Period10/12/0910/14/09

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