"On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media

Monique Chan-Huot; Philippe Lesot; Philippe Pelupessy; Luminita Duma; Geoffrey Bodenhausen; Patricia Duchambon; Michael D. Toney; U. Venkateswara Reddy; N. Suryaprakash

doi:10.1021/ac4004002

"On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media

Monique Chan-Huot
, Philippe Lesot
, Philippe Pelupessy
, Luminita Duma
, Geoffrey Bodenhausen
, Patricia Duchambon
, Michael D. Toney
, U. Venkateswara Reddy
, N. Suryaprakash

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We report the in situ and real-time monitoring of the interconversion of l- and d-alanine-d₃ by alanine racemase from Bacillus stearothermophilus directly observed by ²H NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their ²H quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis-Menten model.

Original language	English (US)
Pages (from-to)	4694-4697
Number of pages	4
Journal	Analytical Chemistry
Volume	85
Issue number	9
DOIs	https://doi.org/10.1021/ac4004002
State	Published - May 7 2013
Externally published	Yes

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10.1021/ac4004002

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title = "{"}On-the-fly{"} kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media",

abstract = "We report the in situ and real-time monitoring of the interconversion of l- and d-alanine-d3 by alanine racemase from Bacillus stearothermophilus directly observed by 2H NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their 2H quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis-Menten model.",

author = "Monique Chan-Huot and Philippe Lesot and Philippe Pelupessy and Luminita Duma and Geoffrey Bodenhausen and Patricia Duchambon and Toney, \{Michael D.\} and Reddy, \{U. Venkateswara\} and N. Suryaprakash",

year = "2013",

month = may,

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doi = "10.1021/ac4004002",

language = "English (US)",

volume = "85",

pages = "4694--4697",

journal = "Analytical Chemistry",

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T1 - "On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media

AU - Chan-Huot, Monique

AU - Lesot, Philippe

AU - Pelupessy, Philippe

AU - Duma, Luminita

AU - Bodenhausen, Geoffrey

AU - Duchambon, Patricia

AU - Toney, Michael D.

AU - Reddy, U. Venkateswara

AU - Suryaprakash, N.

PY - 2013/5/7

Y1 - 2013/5/7

N2 - We report the in situ and real-time monitoring of the interconversion of l- and d-alanine-d3 by alanine racemase from Bacillus stearothermophilus directly observed by 2H NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their 2H quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis-Menten model.

AB - We report the in situ and real-time monitoring of the interconversion of l- and d-alanine-d3 by alanine racemase from Bacillus stearothermophilus directly observed by 2H NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their 2H quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis-Menten model.

UR - https://www.scopus.com/pages/publications/84877355478

UR - https://www.scopus.com/pages/publications/84877355478#tab=citedBy

U2 - 10.1021/ac4004002

DO - 10.1021/ac4004002

M3 - Article

C2 - 23547756

AN - SCOPUS:84877355478

SN - 0003-2700

VL - 85

SP - 4694

EP - 4697

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 9

ER -

"On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media

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