TY - JOUR
T1 - "On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media
AU - Chan-Huot, Monique
AU - Lesot, Philippe
AU - Pelupessy, Philippe
AU - Duma, Luminita
AU - Bodenhausen, Geoffrey
AU - Duchambon, Patricia
AU - Toney, Michael D.
AU - Reddy, U. Venkateswara
AU - Suryaprakash, N.
PY - 2013/5/7
Y1 - 2013/5/7
N2 - We report the in situ and real-time monitoring of the interconversion of l- and d-alanine-d3 by alanine racemase from Bacillus stearothermophilus directly observed by 2H NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their 2H quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis-Menten model.
AB - We report the in situ and real-time monitoring of the interconversion of l- and d-alanine-d3 by alanine racemase from Bacillus stearothermophilus directly observed by 2H NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their 2H quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis-Menten model.
UR - http://www.scopus.com/inward/record.url?scp=84877355478&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877355478&partnerID=8YFLogxK
U2 - 10.1021/ac4004002
DO - 10.1021/ac4004002
M3 - Article
C2 - 23547756
AN - SCOPUS:84877355478
SN - 0003-2700
VL - 85
SP - 4694
EP - 4697
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 9
ER -