On laser scanning confocal microscopy and three dimensional volume rendering of biological structures

Stephen Paddock, Peter DeVries, Jon Holy, Gerald Schatten

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Confocal laser scanning microscopy (CLSM) is a significant improvement over conventional epifluorescence microscopy for observing biological structures. In addition to the increase in resolution and reduction of stray light by CLSM, the serial optical sections of fluorescently-labelled structures produced by CLSM are suitable for computer rendering techniques to produce three dimensional (3D) images of biological structure in the light microscope. The collection and properties of data sets obtained by CLSM and their subsequent computer rendering are described and the biological application of the technology is discussed and illustrated by reconstructions of fluorescently-labelled nuclei and mitotic spindles.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsLouis C. Smith
PublisherPubl by Int Soc for Optical Engineering
Pages20-28
Number of pages9
ISBN (Print)081940246X
StatePublished - Dec 1 1990
EventBioimaging and Two-Dimensional Spectroscopy - Los Angeles, CA, USA
Duration: Jan 18 1990Jan 19 1990

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1205
ISSN (Print)0277-786X

Conference

ConferenceBioimaging and Two-Dimensional Spectroscopy
CityLos Angeles, CA, USA
Period1/18/901/19/90

Fingerprint Dive into the research topics of 'On laser scanning confocal microscopy and three dimensional volume rendering of biological structures'. Together they form a unique fingerprint.

Cite this