Odijk Excluded Volume Interactions during the Unfolding of DNA Confined in a Nanochannel

Jeffrey G. Reifenberger, Han Cao, Kevin D. Dorfman

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8 Scopus citations

Abstract

We report experimental data on the unfolding of human and E. coli genomic DNA molecules shortly after injection into a 45 nm nanochannel. The unfolding dynamics are deterministic, consistent with previous experiments and modeling in larger channels, and do not depend on the biological origin of the DNA. The measured entropic unfolding force per friction per unit contour length agrees with that predicted by combining the Odijk excluded volume with numerical calculations of the Kirkwood diffusivity of confined DNA. The time scale emerging from our analysis has implications for genome mapping in nanochannels, especially as the technology moves toward longer DNA, by setting a lower bound for the delay time before making a measurement.

Original languageEnglish (US)
Pages (from-to)1172-1180
Number of pages9
JournalMacromolecules
Volume51
Issue number3
DOIs
StatePublished - Feb 13 2018

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  • Journal Article

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