Identificaciôn del virus del rayado del banano en Venezuela

Translated title of the contribution: Occurrence of banana streak virus in Venezuela

Mario José Garrido, Alfonso Ordosgoitti, Benham E L Lockhart

Research output: Contribution to journalArticle

Abstract

In banana and plantain (Musa spp.) production areas in Venezuela plants were observed with foliar symptoms of a virus-like disease characterized by chlorotic streaks which often become progressively necrotic. In samples of the cultivars Pineo gigante (AAA) and Mysore (AAB), the banana streak virus (BSV) was detected by immunosorbent electron microscopy. Four virus isolates were identified by immunocapture followed by polymerase chain reaction amplification (IC-PCR) using isolate-specific oligonucleotide primers of two BSV strains. The results of IC-PCR indicated that the isolates occurring in the samples were BSV-OL. This strain is known to be integrated in the host genome (Musa) and there is evidence that BSV infection can arise from these integrated sequences. BSV was detected in the Venezuelan states of Aragua, Barinas, Carabobo, Delta Amacuro, Mérida, Miranda, Sucre, Yaracuy and Zulia.

Original languageSpanish
Pages (from-to)97-101
Number of pages5
JournalInterciencia
Volume30
Issue number2
StatePublished - Feb 1 2005

Fingerprint

Banana streak virus complex
Venezuela
Musa
plantains (fruit)
viruses
DNA primers
bananas
signs and symptoms (plants)
electron microscopy
polymerase chain reaction
sampling
genome
cultivars
infection

Cite this

Identificaciôn del virus del rayado del banano en Venezuela. / Garrido, Mario José; Ordosgoitti, Alfonso; Lockhart, Benham E L.

In: Interciencia, Vol. 30, No. 2, 01.02.2005, p. 97-101.

Research output: Contribution to journalArticle

Garrido, MJ, Ordosgoitti, A & Lockhart, BEL 2005, 'Identificaciôn del virus del rayado del banano en Venezuela', Interciencia, vol. 30, no. 2, pp. 97-101.
Garrido, Mario José ; Ordosgoitti, Alfonso ; Lockhart, Benham E L. / Identificaciôn del virus del rayado del banano en Venezuela. In: Interciencia. 2005 ; Vol. 30, No. 2. pp. 97-101.
@article{87b0d8eda73945c888ddcc5da6b273df,
title = "Identificaci{\^o}n del virus del rayado del banano en Venezuela",
abstract = "In banana and plantain (Musa spp.) production areas in Venezuela plants were observed with foliar symptoms of a virus-like disease characterized by chlorotic streaks which often become progressively necrotic. In samples of the cultivars Pineo gigante (AAA) and Mysore (AAB), the banana streak virus (BSV) was detected by immunosorbent electron microscopy. Four virus isolates were identified by immunocapture followed by polymerase chain reaction amplification (IC-PCR) using isolate-specific oligonucleotide primers of two BSV strains. The results of IC-PCR indicated that the isolates occurring in the samples were BSV-OL. This strain is known to be integrated in the host genome (Musa) and there is evidence that BSV infection can arise from these integrated sequences. BSV was detected in the Venezuelan states of Aragua, Barinas, Carabobo, Delta Amacuro, M{\'e}rida, Miranda, Sucre, Yaracuy and Zulia.",
author = "Garrido, {Mario Jos{\'e}} and Alfonso Ordosgoitti and Lockhart, {Benham E L}",
year = "2005",
month = "2",
day = "1",
language = "Spanish",
volume = "30",
pages = "97--101",
journal = "Interciencia",
issn = "0378-1844",
publisher = "Interciencia Association",
number = "2",

}

TY - JOUR

T1 - Identificaciôn del virus del rayado del banano en Venezuela

AU - Garrido, Mario José

AU - Ordosgoitti, Alfonso

AU - Lockhart, Benham E L

PY - 2005/2/1

Y1 - 2005/2/1

N2 - In banana and plantain (Musa spp.) production areas in Venezuela plants were observed with foliar symptoms of a virus-like disease characterized by chlorotic streaks which often become progressively necrotic. In samples of the cultivars Pineo gigante (AAA) and Mysore (AAB), the banana streak virus (BSV) was detected by immunosorbent electron microscopy. Four virus isolates were identified by immunocapture followed by polymerase chain reaction amplification (IC-PCR) using isolate-specific oligonucleotide primers of two BSV strains. The results of IC-PCR indicated that the isolates occurring in the samples were BSV-OL. This strain is known to be integrated in the host genome (Musa) and there is evidence that BSV infection can arise from these integrated sequences. BSV was detected in the Venezuelan states of Aragua, Barinas, Carabobo, Delta Amacuro, Mérida, Miranda, Sucre, Yaracuy and Zulia.

AB - In banana and plantain (Musa spp.) production areas in Venezuela plants were observed with foliar symptoms of a virus-like disease characterized by chlorotic streaks which often become progressively necrotic. In samples of the cultivars Pineo gigante (AAA) and Mysore (AAB), the banana streak virus (BSV) was detected by immunosorbent electron microscopy. Four virus isolates were identified by immunocapture followed by polymerase chain reaction amplification (IC-PCR) using isolate-specific oligonucleotide primers of two BSV strains. The results of IC-PCR indicated that the isolates occurring in the samples were BSV-OL. This strain is known to be integrated in the host genome (Musa) and there is evidence that BSV infection can arise from these integrated sequences. BSV was detected in the Venezuelan states of Aragua, Barinas, Carabobo, Delta Amacuro, Mérida, Miranda, Sucre, Yaracuy and Zulia.

UR - http://www.scopus.com/inward/record.url?scp=15244346527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15244346527&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:15244346527

VL - 30

SP - 97

EP - 101

JO - Interciencia

JF - Interciencia

SN - 0378-1844

IS - 2

ER -