Occurrence and potential transmission of extended-spectrum beta-lactamase-producing extraintestinal pathogenic and enteropathogenic Escherichia coli in domestic dog faeces from Minnesota

Timothy J. Johnson; Joseph R. Armstrong; Brian Johnston; Irene Merino-Velasco; Ivana Jamborova; Randall S. Singer; James R. Johnson; Jeff B. Bender

doi:10.1111/zph.12985

Occurrence and potential transmission of extended-spectrum beta-lactamase-producing extraintestinal pathogenic and enteropathogenic Escherichia coli in domestic dog faeces from Minnesota

Timothy J. Johnson, Joseph R. Armstrong, Brian Johnston, Irene Merino-Velasco, Ivana Jamborova, Randall S. Singer, James R. Johnson, Jeff B. Bender

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Interactions between humans and pets are increasingly valued in western countries, leading to more extensive contact between humans and their pets within households. Although the magnitude of the risk of transfer of Escherichia coli between humans and their companion animals is undefined, that such transmission occurs has been established and warrants attention. This study examined 186 fresh faecal samples from companion dogs visiting 22 municipal dog parks in the Minneapolis/Saint Paul metropolitan area, Minnesota, USA. Samples were processed to isolate 3rd-generation cephalosporin-resistant E. coli, which were further characterized using PCR-based virulence genotyping, antimicrobial susceptibility profiling and whole-genome sequencing. Of the 186 faecal samples, 29% yielded cephalosporin-resistant E. coli, and 2.2% yielded extended-spectrum beta-lactamase producers. Co-resistance to sulfonamides was typical (77.3% of isolates), and multidrug resistance (i.e. to ≥3 antimicrobial classes), including to combinations of tetracyclines, phenicols, quinolones and aminoglycosides, was substantial (18.9% of isolates). Identified beta-lactamase genes included bla_CMY-2, bla_TEM-1B, bla_TEM-1, bla_CTX-M-24, bla_CTX-M-15 and bla_OXA-1. Genome sequencing of 14 isolates identified genes typical of extraintestinal pathogenic E. coli or enteropathogenic E. coli. In three instances, closely related isolates were recovered from different dogs, within either the same park—suggesting transfer of E. coli between dogs within the park—or different parks—suggesting that dogs may be pre-disposed to carry certain E. coli types, such as those from serogroups O4, O71 and O157. This study adds to the existing evidence that companion dogs can harbour and share antimicrobial-resistant E. coli with presumed intestinal or extraintestinal pathogenic potential.

Original language	English (US)
Pages (from-to)	888-895
Number of pages	8
Journal	Zoonoses and Public Health
Volume	69
Issue number	7
DOIs	https://doi.org/10.1111/zph.12985
State	Published - Nov 2022

Bibliographical note

Funding Information:
This project was supported by the Boehringer Ingelheim Veterinary Scholars Program (JA). Bioinformatics was supported using tools available from the Minnesota Supercomputing Institute. It also was supported in part by the Office of Research and Development, Department of Veteran Affairs (JRJ) and by Spanish Network for Research in Infectious Diseases (REIPI) [RD12/0015/0004, RD16/0016/0011]; a grant from the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) [Ayuda a la formación de la SEIMC 2014 to I.M] and co‐financed by the European Development Regional Fund (ERDF), ‘A Way to Achieve Europe’. I.M. is currently supported by a Río Hortega grant [CM18/00157] by Instituto de Salud Carlos III of Spain.

Funding Information:
This project was supported by the Boehringer Ingelheim Veterinary Scholars Program (JA). Bioinformatics was supported using tools available from the Minnesota Supercomputing Institute. It also was supported in part by the Office of Research and Development, Department of Veteran Affairs (JRJ) and by Spanish Network for Research in Infectious Diseases (REIPI) [RD12/0015/0004, RD16/0016/0011]; a grant from the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) [Ayuda a la formación de la SEIMC 2014 to I.M] and co-financed by the European Development Regional Fund (ERDF), ‘A Way to Achieve Europe’. I.M. is currently supported by a Río Hortega grant [CM18/00157] by Instituto de Salud Carlos III of Spain.

Publisher Copyright:
© 2022 The Authors. Zoonoses and Public Health published by Wiley-VCH GmbH.

Keywords

Escherichia coli
canine
cephalosporin
faecal

Publisher link

10.1111/zph.12985

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Johnson, T. J., Armstrong, J. R., Johnston, B., Merino-Velasco, I., Jamborova, I., Singer, R. S., Johnson, J. R., & Bender, J. B. (2022). Occurrence and potential transmission of extended-spectrum beta-lactamase-producing extraintestinal pathogenic and enteropathogenic Escherichia coli in domestic dog faeces from Minnesota. Zoonoses and Public Health, 69(7), 888-895. https://doi.org/10.1111/zph.12985

Occurrence and potential transmission of extended-spectrum beta-lactamase-producing extraintestinal pathogenic and enteropathogenic Escherichia coli in domestic dog faeces from Minnesota. / Johnson, Timothy J.; Armstrong, Joseph R.; Johnston, Brian et al.
In: Zoonoses and Public Health, Vol. 69, No. 7, 11.2022, p. 888-895.

Research output: Contribution to journal › Article › peer-review

Johnson, TJ, Armstrong, JR, Johnston, B, Merino-Velasco, I, Jamborova, I, Singer, RS , Johnson, JR & Bender, JB 2022, 'Occurrence and potential transmission of extended-spectrum beta-lactamase-producing extraintestinal pathogenic and enteropathogenic Escherichia coli in domestic dog faeces from Minnesota', Zoonoses and Public Health, vol. 69, no. 7, pp. 888-895. https://doi.org/10.1111/zph.12985

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note = "Funding Information: This project was supported by the Boehringer Ingelheim Veterinary Scholars Program (JA). Bioinformatics was supported using tools available from the Minnesota Supercomputing Institute. It also was supported in part by the Office of Research and Development, Department of Veteran Affairs (JRJ) and by Spanish Network for Research in Infectious Diseases (REIPI) [RD12/0015/0004, RD16/0016/0011]; a grant from the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) [Ayuda a la formaci{\'o}n de la SEIMC 2014 to I.M] and co‐financed by the European Development Regional Fund (ERDF), {\textquoteleft}A Way to Achieve Europe{\textquoteright}. I.M. is currently supported by a R{\'i}o Hortega grant [CM18/00157] by Instituto de Salud Carlos III of Spain. Funding Information: This project was supported by the Boehringer Ingelheim Veterinary Scholars Program (JA). Bioinformatics was supported using tools available from the Minnesota Supercomputing Institute. It also was supported in part by the Office of Research and Development, Department of Veteran Affairs (JRJ) and by Spanish Network for Research in Infectious Diseases (REIPI) [RD12/0015/0004, RD16/0016/0011]; a grant from the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) [Ayuda a la formaci{\'o}n de la SEIMC 2014 to I.M] and co-financed by the European Development Regional Fund (ERDF), {\textquoteleft}A Way to Achieve Europe{\textquoteright}. I.M. is currently supported by a R{\'i}o Hortega grant [CM18/00157] by Instituto de Salud Carlos III of Spain. Publisher Copyright: {\textcopyright} 2022 The Authors. Zoonoses and Public Health published by Wiley-VCH GmbH.",

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Occurrence and potential transmission of extended-spectrum beta-lactamase-producing extraintestinal pathogenic and enteropathogenic Escherichia coli in domestic dog faeces from Minnesota

Abstract

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