Observations on the Chromatographic Heterogeneity of Normal Adult and Fetal Human Hemoglobin: A Study of the Effects of Crystallization and Chromatography on the Heterogeneity and Isoleucine Content

David W Allen, W. A. Schroeder, Joan Balog

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236 Citations (Scopus)

Abstract

By chromatographic methods, it has been shown that both crystallized and uncrystallized normal adult human hemoglobin contain a main component that comprises about 90% of the heme proteins. Three minor heme proteins and a non-heme protein also have been detected. The several components are not artifacts of preparation or chromatography, and they have been shown to differ in their content of isoleucine. Cord blood hemoglobin also contains minor components. These results are discussed in relation to other reports of the heterogeneity of human hemoglobin. The methods described may be used for the isolation of preparative amounts of the main components.

Original languageEnglish (US)
Pages (from-to)1628-1634
Number of pages7
JournalJournal of the American Chemical Society
Volume80
Issue number7
DOIs
StatePublished - Apr 1 1958

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Fetal Hemoglobin
Isoleucine
Hemoglobin
Crystallization
Chromatography
Hemeproteins
Hemoglobins
Fetal Blood
Artifacts
Blood
Proteins

Cite this

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abstract = "By chromatographic methods, it has been shown that both crystallized and uncrystallized normal adult human hemoglobin contain a main component that comprises about 90{\%} of the heme proteins. Three minor heme proteins and a non-heme protein also have been detected. The several components are not artifacts of preparation or chromatography, and they have been shown to differ in their content of isoleucine. Cord blood hemoglobin also contains minor components. These results are discussed in relation to other reports of the heterogeneity of human hemoglobin. The methods described may be used for the isolation of preparative amounts of the main components.",
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T1 - Observations on the Chromatographic Heterogeneity of Normal Adult and Fetal Human Hemoglobin

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AU - Allen, David W

AU - Schroeder, W. A.

AU - Balog, Joan

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Y1 - 1958/4/1

N2 - By chromatographic methods, it has been shown that both crystallized and uncrystallized normal adult human hemoglobin contain a main component that comprises about 90% of the heme proteins. Three minor heme proteins and a non-heme protein also have been detected. The several components are not artifacts of preparation or chromatography, and they have been shown to differ in their content of isoleucine. Cord blood hemoglobin also contains minor components. These results are discussed in relation to other reports of the heterogeneity of human hemoglobin. The methods described may be used for the isolation of preparative amounts of the main components.

AB - By chromatographic methods, it has been shown that both crystallized and uncrystallized normal adult human hemoglobin contain a main component that comprises about 90% of the heme proteins. Three minor heme proteins and a non-heme protein also have been detected. The several components are not artifacts of preparation or chromatography, and they have been shown to differ in their content of isoleucine. Cord blood hemoglobin also contains minor components. These results are discussed in relation to other reports of the heterogeneity of human hemoglobin. The methods described may be used for the isolation of preparative amounts of the main components.

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