Observation of vascular injury after freezing: investigating the response of normal skin and subcutaneous AT-1 tumor tissue to cryosurgery in the dorsal skin flap chamber

Nathan E. Hoffmann; David J. Swanlund; John C. Bischof

doi:10.1115/IMECE1999-0579

Observation of vascular injury after freezing: investigating the response of normal skin and subcutaneous AT-1 tumor tissue to cryosurgery in the dorsal skin flap chamber

Nathan E. Hoffmann, David J. Swanlund, John C. Bischof

Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

A study was performed to test the hypothesis that host response injury, specifically vascular injury, causes the majority of tissue necrosis at the edge of a frozen region and therefore determines the size of the lesion seen after in vivo freezing. The dorsal skin flap chamber (DSFC) implanted in the Copenhagen rat served as the cryosurgical model. There was a statistical correlation between the region of vascular stasis and the region of tissue necrosis, and the lesion sizes were statistically similar for tumor and normal tissue which are dissimilar save for the presence of a vasculature. The minimum temperature required to cause this damage was much higher than the temperature required for AT-1 cell destruction in vitro, and was similar to temperatures which caused vascular injury in other studies. These results lend support to the addressed hypothesis.

Original language	English (US)
Title of host publication	Advances in Heat and Mass Transfer in Biotechnology
Publisher	American Society of Mechanical Engineers (ASME)
Pages	39-41
Number of pages	3
Volume	44
ISBN (Electronic)	9780791816431
ISBN (Print)	0791816435
DOIs	https://doi.org/10.1115/IMECE1999-0579
State	Published - Dec 1 1999
Event	ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999 - Nashville, United States Duration: Nov 14 1999 → Nov 19 1999

Publication series

Name	ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume	1999-E

Conference

Conference	ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999
Country/Territory	United States
City	Nashville
Period	11/14/99 → 11/19/99

Bibliographical note

Funding Information:
This work was supported by the NIH 1R29-CA75284-01 Al, the Department of Biomedical Engineering at the U of M and a gift from CANDELA Corporation.

Publisher link

10.1115/IMECE1999-0579

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Hoffmann, N. E., Swanlund, D. J., & Bischof, J. C. (1999). Observation of vascular injury after freezing: investigating the response of normal skin and subcutaneous AT-1 tumor tissue to cryosurgery in the dorsal skin flap chamber. In Advances in Heat and Mass Transfer in Biotechnology (Vol. 44, pp. 39-41). (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 1999-E). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE1999-0579

Observation of vascular injury after freezing: investigating the response of normal skin and subcutaneous AT-1 tumor tissue to cryosurgery in the dorsal skin flap chamber. / Hoffmann, Nathan E.; Swanlund, David J.; Bischof, John C.
Advances in Heat and Mass Transfer in Biotechnology. Vol. 44 American Society of Mechanical Engineers (ASME), 1999. p. 39-41 (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 1999-E).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hoffmann, NE, Swanlund, DJ & Bischof, JC 1999, Observation of vascular injury after freezing: investigating the response of normal skin and subcutaneous AT-1 tumor tissue to cryosurgery in the dorsal skin flap chamber. in Advances in Heat and Mass Transfer in Biotechnology. vol. 44, ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), vol. 1999-E, American Society of Mechanical Engineers (ASME), pp. 39-41, ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999, Nashville, United States, 11/14/99. https://doi.org/10.1115/IMECE1999-0579

Hoffmann NE, Swanlund DJ, Bischof JC. Observation of vascular injury after freezing: investigating the response of normal skin and subcutaneous AT-1 tumor tissue to cryosurgery in the dorsal skin flap chamber. In Advances in Heat and Mass Transfer in Biotechnology. Vol. 44. American Society of Mechanical Engineers (ASME). 1999. p. 39-41. (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)). doi: 10.1115/IMECE1999-0579

Hoffmann, Nathan E. ; Swanlund, David J. ; Bischof, John C. / Observation of vascular injury after freezing : investigating the response of normal skin and subcutaneous AT-1 tumor tissue to cryosurgery in the dorsal skin flap chamber. Advances in Heat and Mass Transfer in Biotechnology. Vol. 44 American Society of Mechanical Engineers (ASME), 1999. pp. 39-41 (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)).

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abstract = "A study was performed to test the hypothesis that host response injury, specifically vascular injury, causes the majority of tissue necrosis at the edge of a frozen region and therefore determines the size of the lesion seen after in vivo freezing. The dorsal skin flap chamber (DSFC) implanted in the Copenhagen rat served as the cryosurgical model. There was a statistical correlation between the region of vascular stasis and the region of tissue necrosis, and the lesion sizes were statistically similar for tumor and normal tissue which are dissimilar save for the presence of a vasculature. The minimum temperature required to cause this damage was much higher than the temperature required for AT-1 cell destruction in vitro, and was similar to temperatures which caused vascular injury in other studies. These results lend support to the addressed hypothesis.",

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Observation of vascular injury after freezing: investigating the response of normal skin and subcutaneous AT-1 tumor tissue to cryosurgery in the dorsal skin flap chamber

Abstract

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