A study was performed to test the hypothesis that host response injury, specifically vascular injury, causes the majority of tissue necrosis at the edge of a frozen region and therefore determines the size of the lesion seen after in vivo freezing. The dorsal skin flap chamber (DSFC) implanted in the Copenhagen rat served as the cryosurgical model. There was a statistical correlation between the region of vascular stasis and the region of tissue necrosis, and the lesion sizes were statistically similar for tumor and normal tissue which are dissimilar save for the presence of a vasculature. The minimum temperature required to cause this damage was much higher than the temperature required for AT-1 cell destruction in vitro, and was similar to temperatures which caused vascular injury in other studies. These results lend support to the addressed hypothesis.
|Original language||English (US)|
|Title of host publication||Advances in Heat and Mass Transfer in Biotechnology|
|Publisher||American Society of Mechanical Engineers (ASME)|
|Number of pages||3|
|State||Published - Dec 1 1999|
|Event||ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999 - Nashville, United States|
Duration: Nov 14 1999 → Nov 19 1999
|Name||ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)|
|Conference||ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999|
|Period||11/14/99 → 11/19/99|
Bibliographical noteFunding Information:
This work was supported by the NIH 1R29-CA75284-01 Al, the Department of Biomedical Engineering at the U of M and a gift from CANDELA Corporation.