Objectives: To evaluate the laboratory diagnosis aspects of obesity-related health problems with special reference to postmenopausal breast cancer. Design and methods: We conducted a systemic search of the literature primarily from the PubMed to obtain the relevant data. Results: Obesity is associated with the dysregulations of a number of body components such as blood constituents, extracellular matrix, and hormones/growth factors axes, which could be utilized for early diagnosis. Conclusions: Obesity-related disorders including breast cancer have emerged as major health problems in almost all the nations. There is a need to elucidate different biochemical markers that are being used in the clinics or have the potential for such use. A precise understanding of the complex pathologies related with obesity is useful in prevention, early diagnosis and overall clinical management.