TY - JOUR
T1 - O-GlcNAcylation of master growth repressor DELLA by SECRET AGENT modulates multiple signaling pathways in Arabidopsis
AU - Zentella, Rodolfo
AU - Hu, Jianhong
AU - Hsieh, Wen Ping
AU - Matsumoto, Peter A.
AU - Dawdy, Andrew
AU - Barnhill, Benjamin
AU - Oldenhof, Harriëtte
AU - Hartweck, Lynn M.
AU - Maitra, Sushmit
AU - Thomas, Stephen G.
AU - Cockrell, Shelley
AU - Boyce, Michael
AU - Shabanowitz, Jeffrey
AU - Hunt, Donald F.
AU - Olszewski, Neil E.
AU - Sun, Tai Ping
N1 - Publisher Copyright:
© 2016 Zentella et al.
PY - 2016/1/15
Y1 - 2016/1/15
N2 - The DELLA family of transcription regulators functions as master growth repressors in plants by inhibiting phytohormone gibberellin (GA) signaling in response to developmental and environmental cues. DELLAs also play a central role in mediating cross-talk between GA and other signaling pathways via antagonistic direct interactions with key transcription factors. However, how these crucial protein-protein interactions can be dynamically regulated during plant development remains unclear. Here, we show that DELLAs are modified by the O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) SECRET AGENT (SEC) in Arabidopsis. O-GlcNAcylation of the DELLA protein REPRESSOR OF ga1-3 (RGA) inhibits RGA binding to four of its interactors-PHYTOCHROME-INTERACTING FACTOR3 (PIF3), PIF4, JASMONATE-ZIM DOMAIN1, and BRASSINAZOLERESISTANT1 (BZR1)—that are key regulators in light, jasmonate, and brassinosteroid signaling pathways, respectively. Consistent with this, the sec-null mutant displayed reduced responses to GA and brassinosteroid and showed decreased expression of several common target genes of DELLAs, BZR1, and PIFs. Our results reveal a direct role of OGT in repressing DELLA activity and indicate that O-GlcNAcylation of DELLAs provides a fine-tuning mechanism in coordinating multiple signaling activities during plant development.
AB - The DELLA family of transcription regulators functions as master growth repressors in plants by inhibiting phytohormone gibberellin (GA) signaling in response to developmental and environmental cues. DELLAs also play a central role in mediating cross-talk between GA and other signaling pathways via antagonistic direct interactions with key transcription factors. However, how these crucial protein-protein interactions can be dynamically regulated during plant development remains unclear. Here, we show that DELLAs are modified by the O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) SECRET AGENT (SEC) in Arabidopsis. O-GlcNAcylation of the DELLA protein REPRESSOR OF ga1-3 (RGA) inhibits RGA binding to four of its interactors-PHYTOCHROME-INTERACTING FACTOR3 (PIF3), PIF4, JASMONATE-ZIM DOMAIN1, and BRASSINAZOLERESISTANT1 (BZR1)—that are key regulators in light, jasmonate, and brassinosteroid signaling pathways, respectively. Consistent with this, the sec-null mutant displayed reduced responses to GA and brassinosteroid and showed decreased expression of several common target genes of DELLAs, BZR1, and PIFs. Our results reveal a direct role of OGT in repressing DELLA activity and indicate that O-GlcNAcylation of DELLAs provides a fine-tuning mechanism in coordinating multiple signaling activities during plant development.
KW - DELLA-interacting proteins
KW - Gibberellin signaling
KW - Hormone signaling
KW - O-GlcNAc transferase
KW - O-GlcNAcylation of DELLA
KW - SECRET AGENTs
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U2 - 10.1101/gad.270587.115
DO - 10.1101/gad.270587.115
M3 - Article
C2 - 26773002
AN - SCOPUS:84954553651
SN - 0890-9369
VL - 30
SP - 164
EP - 176
JO - Genes and Development
JF - Genes and Development
IS - 2
ER -