Nucleotide sequence of the open reading frame (ORF) for the phosphoenolpyruvate carboxylase gene (ppc) of the cyanobacterium Anacystis nidulans was determined. The ORF consists of 3159 bp and codes for 1053 amino acid (aa) residues. The codon usage of the ppc of A. nidulans is not so markedly different from that of the Escherichia colippc, yet, in A. nidulans the preferred codons are AAG for lysine and CCC for proline, whereas those are seldom used in the E. coli ppc.
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We would like to expresso ur sinceret hankst o Drs. M. Takanamia nd T. Takeya (Kyoto University) for the instructiono f nt sequencintge chnique. Thanks are also due to Dr. T. Katoh (Kyoto University) for his useful suggestionds uring this work. This work was supportedin partsb y Grants-in-Aid for ScientificR esearchf rom the Ministry of Education, Science and Culture of Japan, Grant from the Nissan ScienceF oundationa nd by Grant from SumitomoC hemicalC o. Ltd.
- Recombinant DNA
- codon usage
- photosynthetic CO