Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor

Paulraj Kirubakaran Lawrence, Jayaveeramuthu Nirmala, Kripa Ram Koundal

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A protease inhibitor gene has been isolated from native cowpea (Vigna unguiculata L.) cv. 130 from λ-ZAP II genomic library. The nucleotide sequence of this genomic clone shared 86% homology with a cowpea trypsin inhibitor f IV mRNA, and 81% with Bowman-Birk protease inhibitor genes of soybean. The isolated gene had TATA and CAT signals in the 5′ upstream region. The longest open reading frame had 504 bases, encoding 167 amino acids from the predicted coding region. The active serine centers of this protein was found between 105-158 amino acid residues, with a 69 amino acids long signal peptide in its N terminal.

Original languageEnglish (US)
Pages (from-to)46-51
Number of pages6
JournalElectronic Journal of Biotechnology
Volume4
Issue number1
StatePublished - 2001

Keywords

  • Cowpea genomic library
  • Nucleotide sequencing
  • Sequence analysis

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