TY - JOUR
T1 - Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor
AU - Lawrence, Paulraj Kirubakaran
AU - Nirmala, Jayaveeramuthu
AU - Koundal, Kripa Ram
PY - 2001
Y1 - 2001
N2 - A protease inhibitor gene has been isolated from native cowpea (Vigna unguiculata L.) cv. 130 from λ-ZAP II genomic library. The nucleotide sequence of this genomic clone shared 86% homology with a cowpea trypsin inhibitor f IV mRNA, and 81% with Bowman-Birk protease inhibitor genes of soybean. The isolated gene had TATA and CAT signals in the 5′ upstream region. The longest open reading frame had 504 bases, encoding 167 amino acids from the predicted coding region. The active serine centers of this protein was found between 105-158 amino acid residues, with a 69 amino acids long signal peptide in its N terminal.
AB - A protease inhibitor gene has been isolated from native cowpea (Vigna unguiculata L.) cv. 130 from λ-ZAP II genomic library. The nucleotide sequence of this genomic clone shared 86% homology with a cowpea trypsin inhibitor f IV mRNA, and 81% with Bowman-Birk protease inhibitor genes of soybean. The isolated gene had TATA and CAT signals in the 5′ upstream region. The longest open reading frame had 504 bases, encoding 167 amino acids from the predicted coding region. The active serine centers of this protein was found between 105-158 amino acid residues, with a 69 amino acids long signal peptide in its N terminal.
KW - Cowpea genomic library
KW - Nucleotide sequencing
KW - Sequence analysis
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M3 - Article
AN - SCOPUS:0042755411
SN - 0717-3458
VL - 4
SP - 46
EP - 51
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
IS - 1
ER -