Nucleotide sequence and molecular characterization of a maize mitochondrial plasmid-like DNA

Alan G. Smith, Daryl R. Pring

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26 Scopus citations

Abstract

The mitochondrial genome of Black Mexican Sweet maize consists of the principal genome, a 2.3 kb minilinear DNA, a 1,913 by (1.9 kb) and a 1,445 bp (1.4 kb) minicircular DNA. The three extrachromosomal DNAs exhibit characteristics of autonomous replication in cell suspension culture. The complete sequence of the 1.4 kb minicircle was determined. It has 61 by of near perfect sequence homology to the 1.9 kb minicircle. Both minicircular DNAs are transcriptionally active; the longest open reading frame of the 1.4 kb minicircle was 231 bp. A putative origin of replication was identified as a high A+T sequence. These minicircles were present in some but not all of 20 maize lines surveyed. None of the lines examined carried the 1.4 kb minicircle without the 1.9 kb minicircle. Nuclear DNA of one line of the seven examined carried homology to both DNAs.

Original languageEnglish (US)
Pages (from-to)617-623
Number of pages7
JournalCurrent Genetics
Volume12
Issue number8
DOIs
StatePublished - Dec 1987

Keywords

  • A+T rich
  • Distribution
  • Nuclear homology
  • Transcription

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