Nucleic acid binding and chaperone properties of HIV-1 Gag and nucleocapsid proteins

Margareta Cruceanu, Maria A. Urbaneja, Catherine V. Hixson, Donald G. Johnson, Siddhartha A. Datta, Matthew J. Fivash, Andrew G. Stephen, Robert J. Fisher, Robert J. Gorelick, Jose R. Casas-Finet, Alan Rein, Ioulia Rouzina, Mark C. Williams

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

The Gag polyprotein of HIV-1 is essential for retroviral replication and packaging. The nucleocapsid (NC) protein is the primary region for the interaction of Gag with nucleic acids. In this study, we examine the interactions of Gag and its NC cleavage products (NCp15, NCp9 and NCp7) with nucleic acids using solution and single molecule experiments. The NC cleavage products bound DNA with comparable affinity and strongly destabilized the DNA duplex. In contrast, the binding constant of Gag to DNA was found to be ∼10-fold higher than that of the NC proteins, and its destabilizing effect on dsDNA was negligible. These findings are consistent with the primary function of Gag as a nucleic acid binding and packaging protein and the primary function of the NC proteins as nucleic acid chaperones. Also, our results suggest that NCp7's capability for fast sequence-nonspecific nucleic acid duplex destabilization, as well as its ability to facilitate nucleic acid strand annealing by inducing electrostatic attraction between strands, likely optimize the fully processed NC protein to facilitate complex nucleic acid secondary structure rearrangements. In contrast, Gag's stronger DNA binding and aggregation capabilities likely make it an effective chaperone for processes that do not require significant duplex destabilization.

Original languageEnglish (US)
Pages (from-to)593-605
Number of pages13
JournalNucleic acids research
Volume34
Issue number2
DOIs
StatePublished - Feb 2006

Bibliographical note

Funding Information:
This work was funded in part by the National Cancer Institute under contract No. NO1-CO-12400 with SAIC-Frederick, Inc., by the National Science Foundation (MCB-0238190), the National Institutes of Health (GM-072462), by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research, and the Research Corporation. M.A.U. was partially funded by a fellowship for Perfeccionamiento y Movilidad del Personal Investi-gador from the Departamento de Educación, Universidades e Investigación del Gobierno Vasco (Spain). We thank Prof. Karin Musier-Forsyth for helpful discussions and Prof. Richard L. Karpel for a critical reading of the manuscript. Funding to pay the Open Access publication charges for this article was provided by NIH GM-072462.

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