Nuclear—organelle interactions

the immutans variegation mutant of Arabidopsis is plastid autonomous and impaired in carotenoid biosynthesis

Carolyn M. Wetzel, Cai‐Zhong ‐Z Jiang, Le Ann J. Meehan, Daniel F. Voytas, Steven R. Rodermel

Research output: Contribution to journalArticle

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Abstract

The immutans (im) variegation mutant of Arabidopsis thaliana contains green‐ and white‐sectored leaves due to the action of a nuclear recessive gene. The mutation is somatically unstable, and the degree of sectoring is influenced by light and temperature. Whereas the cells in the green sectors contain normal chloroplasts, the cells in the white sectors are heteroplastidic and contain non‐pigmented plastids that lack organized lamellar structures, as well as small pigmented plastids and/or rare normal chloroplasts. This indicates that the plastids in im white cells are not affected equally by the nuclear mutation and that the expression of immutans is ‘plastid autonomous’. In contrast to other variegation mutants with heteroplastidic cells, the defect in im is not maternally inherited. immutans thus represents a novel type of nuclear gene‐induced variegation mutant. It has also been found that the white tissues of immutans accumulate phytoene, a non‐colored C40 carotenoid intermediate. This suggests that immutans controls, either directly or indirectly, the activity of phytoene desaturase (PDS), the enzyme that converts phytoene to zeta‐carotene in higher plants. However, im is not the structural gene for PDS. A secondary effect of carotenoid deficiency, both in immutans and in wild‐type plants treated with a herbicide that blocks carotenoid synthesis, is an increase in acid ribonuclease activity in white tissue. It is concluded that the novel variegation generated by the immutans mutation should offer great insight into the complex circuitry that regulates nuclear—organelle interactions.

Original languageEnglish (US)
Pages (from-to)161-175
Number of pages15
JournalThe Plant Journal
Volume6
Issue number2
DOIs
StatePublished - Jan 1 1994

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Plastids
Carotenoids
Arabidopsis
plastids
carotenoids
biosynthesis
mutants
Chloroplasts
mutation
Mutation
chloroplasts
cells
Recessive Genes
ribonucleases
structural genes
recessive genes
Herbicides
Ribonucleases
Arabidopsis thaliana
herbicides

Cite this

Nuclear—organelle interactions : the immutans variegation mutant of Arabidopsis is plastid autonomous and impaired in carotenoid biosynthesis. / Wetzel, Carolyn M.; Jiang, Cai‐Zhong ‐Z; Meehan, Le Ann J.; Voytas, Daniel F.; Rodermel, Steven R.

In: The Plant Journal, Vol. 6, No. 2, 01.01.1994, p. 161-175.

Research output: Contribution to journalArticle

Wetzel, Carolyn M. ; Jiang, Cai‐Zhong ‐Z ; Meehan, Le Ann J. ; Voytas, Daniel F. ; Rodermel, Steven R. / Nuclear—organelle interactions : the immutans variegation mutant of Arabidopsis is plastid autonomous and impaired in carotenoid biosynthesis. In: The Plant Journal. 1994 ; Vol. 6, No. 2. pp. 161-175.
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