Nuclear microinjection to assess how heterologously expressed proteins impact Ca2+ signals in Xenopus oocytes

Yaping Lin-Moshier, Jonathan S. Marchant

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The Xenopus oocyte is frequently used for heterologous expression and for studying the spatiotemporal patterning of Ca2+ signals. Here, we outline a protocol for nuclear microinjection of the Xenopus oocyte for the purpose of studying how subsequently expressed proteins impact intracellular Ca2+ signals evoked by inositol trisphosphate (InsP3). Injected oocytes can easily be identified by reporter technologies and the impact of heterologously expressed proteins on the generation and properties of InsP3-evoked Ca2+ signals can be resolved using caged InsP3 and fluorescent Ca2+ indicators.

Original languageEnglish (US)
Pages (from-to)255-261
Number of pages7
JournalCold Spring Harbor Protocols
Volume8
Issue number3
DOIs
StatePublished - 2013

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