Nuclear matrix as acceptor for cAMP-binding proteins

Said A. Goueli, Khalil Ahmed

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Using [32P]-8-N3-cAMP, a photoaffinity analog of cAMP, we have established that nuclear binding of cAMP is preferentially localized in the "nuclear matrix". Two major radioactive bands corresponded to proteins of Mr 40 K and 50 K, and three minor bands to proteins of Mr 55, 150 and 200 K. Even though the molecular weight of the major nuclear binding proteins in the matrix are similar to those of the cytosolic cAMP binding proteins, the characteristics of the binding reaction in the nucleus were markedly different from those in the cytosol.

Original languageEnglish (US)
Pages (from-to)1083-1088
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume109
Issue number4
DOIs
StatePublished - Dec 31 1982

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Nuclear Matrix
Carrier Proteins
Nuclear Matrix-Associated Proteins
Protein Binding
Cytosol
Proteins
Molecular Weight
Molecular weight
8-azidoadenosine-3',5'-monophosphate

Cite this

Nuclear matrix as acceptor for cAMP-binding proteins. / Goueli, Said A.; Ahmed, Khalil.

In: Biochemical and Biophysical Research Communications, Vol. 109, No. 4, 31.12.1982, p. 1083-1088.

Research output: Contribution to journalArticle

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