Nuclear Magnetic Resonance Relaxation of Glycogen H1 in Solution

Wei Chen, Xiao Hong Zhu, Robert G. Shulman, Malcolm J. Avison

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16 Scopus citations

Abstract

The NMR relaxation properties of the H1 proton of oyster glycogen in D2O and H2O solutions have been studied using nonselective, semiselective, and selective inversion recovery and Hahn spin-echo pulse sequences. The data were analyzed in terms of an isotropic, rigid-rotor dipole-dipole model including cross-relaxation. At 8.4 T in D2O, ρ = 5.4 ± 0.4 s−1 and σ = −4.5 ± 0.4 s−1. The large, negative σ value is consistent with strong cross-relaxation and a long correlation time. The relaxation data can be explained by a single correlation time, τc = 2.7 × 10−9 s, indicating significant internal mobility. With this value of τc, and assuming that the structure of the glucose moieties was the same as in α-D-glucose crystals, the dipole sum contributing to T1 relaxation was calculated. The intra-ring relaxation was dominated by dipole fields from the H2 proton, but these only accounted for ∼18% of the total relaxation. Most of the relaxation comes from inter-glucose relaxation. From modeling, this is dominated by the H4′ across the α-1,4-glycosidic bond. The H1 longitudinal relaxation rates were significantly enhanced in H2O compared with D2O. This enhancement is not due to direct dipolar interaction between H1 and bulk water. Transverse relaxation rates were not significantly enhanced in H2O.

Original languageEnglish (US)
Pages (from-to)9417-9422
Number of pages6
JournalBiochemistry
Volume32
Issue number36
DOIs
StatePublished - 1993

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