Nuclear magnetic resonance-based quantification of organic diphosphates

Stepan Lenevich, Mark D. Distefano

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Phosphorylated compounds are ubiquitous in life. Given their central role, many such substrates and analogs have been prepared for subsequent evaluation. Prior to biological experiments, it is typically necessary to determine the concentration of the target molecule in solution. Here we describe a method where concentrations of stock solutions of organic diphosphates and bisphosphonates are quantified using 31P nuclear magnetic resonance (NMR) spectroscopy with standard instrumentation using a capillary tube with a secondary standard. The method is specific and is applicable down to a concentration of 200μM. The capillary tube provides the reference peak for quantification and deuterated solvent for locking.

Original languageEnglish (US)
Pages (from-to)316-320
Number of pages5
JournalAnalytical Biochemistry
Issue number2
StatePublished - Jan 15 2011

Bibliographical note

Funding Information:
This research was supported by the National Institutes of Health (Grant GM58442 to M.D.D.).


  • Bisphosphonates
  • Diphosphates
  • NMR
  • Phosphorus analysis
  • Phosphorus quantification
  • Pyrophosphate


Dive into the research topics of 'Nuclear magnetic resonance-based quantification of organic diphosphates'. Together they form a unique fingerprint.

Cite this