Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules

Amy J. Prunuske, Jin Liu, Suzanne Elgort, Jomon Joseph, Mary Dasso, Katharine S. Ullman

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

When higher eukaryotic cells transition into mitosis, the nuclear envelope, nuclear pore complexes, and nuclear lamina are coordinately disassembled. The COPI coatomer complex, which plays a major role in membrane remodeling at the Golgi, has been implicated in the process of nuclear envelope breakdown and requires interactions at the nuclear pore complex for recruitment to this new site of action at mitosis. Nup153, a resident of the nuclear pore basket, was found to be involved in COPI recruitment, but the molecular nature of the interface between COPI and the nuclear pore has not been fully elucidated. To better understand what occurs at the nuclear pore at this juncture, we have probed the role of the nucleoporin Nup358/RanBP2. Nup358 contains a repetitive zinc finger domain with overall organization similar to a region within Nup153 that is critical to COPI association, yet inspection of these two zinc finger domains reveals features that also clearly distinguish them. Here, we found that the Nup358 zinc finger domain, but not a zinc finger domain from an unrelated protein, binds to COPI and dominantly inhibits progression of nuclear envelope breakdown in an assay that robustly recapitulates this process in vitro. Moreover, the Nup358 zinc finger domain interferes with COPI recruitment to the nuclear rim. Consistent with a role for this pore protein in coordinating nuclear envelope breakdown, Nup358-specific antibodies impair nuclear disassembly. Significantly, targeting either Nup153 or Nup358 for inhibition perturbs nuclear envelope breakdown, supporting a model in which these nucleoporins play nonredundant roles, perhaps contributing to COPI recruitment platforms on both the nuclear and cytoplasmic faces of the pore. We found that an individual zinc finger is the minimal interface for COPI association, although tandem zinc fingers are optimal. These results provide new information about the critical components of nuclear membrane remodeling and lay the foundation for a better understanding of how this process is regulated.

Original languageEnglish (US)
Pages (from-to)760-769
Number of pages10
JournalMolecular Biology of the Cell
Volume17
Issue number2
DOIs
StatePublished - Feb 1 2006

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Coat Protein Complex I
Nuclear Pore Complex Proteins
Zinc Fingers
Nuclear Envelope
Nuclear Pore
Mitosis
Nuclear Lamina
Porins
Eukaryotic Cells

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Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. / Prunuske, Amy J.; Liu, Jin; Elgort, Suzanne; Joseph, Jomon; Dasso, Mary; Ullman, Katharine S.

In: Molecular Biology of the Cell, Vol. 17, No. 2, 01.02.2006, p. 760-769.

Research output: Contribution to journalArticle

Prunuske, Amy J. ; Liu, Jin ; Elgort, Suzanne ; Joseph, Jomon ; Dasso, Mary ; Ullman, Katharine S. / Nuclear envelope breakdown is coordinated by both Nup358/RanBP2 and Nup153, two nucleoporins with zinc finger modules. In: Molecular Biology of the Cell. 2006 ; Vol. 17, No. 2. pp. 760-769.
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