NtrC-sensed nitrogen availability is important for oxidative stress defense in Pseudomonas putida KT2440

Sujin Yeom, Jinki Yeom, Woojun Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The zwf, which encodes glucose-6-phosphate dehydrogenase, is repressed by NtrC under nitrogen-limited condition. Previously, we demonstrated that induction of zwf-1 is required for protecting Pseudomonas putida cells under oxidative stress, which could be possible probably because of derepression of HexR on the zwf-1 gene under oxidative stress. These findings led us investigate that NtrC still represses the zwf-1 under nitrogen-limited oxidative stress condition, which makes cells more sensitive under such condition. Interestingly, deletion of the ntrC gene significantly reduces growth rate, but renders cells more resistant to oxidative stress, under nitrogen limited condition in P. putida. More vitality of the ntrC mutant under oxidative stress condition was also confirmed by the fluorogenic redox dye using flow cytometry. The results of transcriptome analysis demonstrated that the derepression of several oxidative stress genes along with the zwf-1 gene might confer high resistance to oxidative stress in the ntrC mutant. Here, we presented the data for the first time, showing that different sets of genes are involved in nitrogen-rich and nitrogen-limited oxidative stress conditions and NtrC-sensed nitrogen availability is one of the most important prerequisite for full cellular defense against oxidative stress in P. putida.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalJournal of Microbiology
Volume48
Issue number2
DOIs
StatePublished - Apr 1 2010

Fingerprint

Pseudomonas putida
Oxidative Stress
Nitrogen
Genes
Glucosephosphate Dehydrogenase
Gene Deletion
Gene Expression Profiling
Oxidation-Reduction
Flow Cytometry
Coloring Agents

Keywords

  • HexR
  • NADPH
  • Nitrogen
  • Soil model bacterium
  • SoxR
  • Zwf-1

Cite this

NtrC-sensed nitrogen availability is important for oxidative stress defense in Pseudomonas putida KT2440. / Yeom, Sujin; Yeom, Jinki; Park, Woojun.

In: Journal of Microbiology, Vol. 48, No. 2, 01.04.2010, p. 153-159.

Research output: Contribution to journalArticle

Yeom, Sujin ; Yeom, Jinki ; Park, Woojun. / NtrC-sensed nitrogen availability is important for oxidative stress defense in Pseudomonas putida KT2440. In: Journal of Microbiology. 2010 ; Vol. 48, No. 2. pp. 153-159.
@article{24f365b3d9bb44aa879ef2196b675ca6,
title = "NtrC-sensed nitrogen availability is important for oxidative stress defense in Pseudomonas putida KT2440",
abstract = "The zwf, which encodes glucose-6-phosphate dehydrogenase, is repressed by NtrC under nitrogen-limited condition. Previously, we demonstrated that induction of zwf-1 is required for protecting Pseudomonas putida cells under oxidative stress, which could be possible probably because of derepression of HexR on the zwf-1 gene under oxidative stress. These findings led us investigate that NtrC still represses the zwf-1 under nitrogen-limited oxidative stress condition, which makes cells more sensitive under such condition. Interestingly, deletion of the ntrC gene significantly reduces growth rate, but renders cells more resistant to oxidative stress, under nitrogen limited condition in P. putida. More vitality of the ntrC mutant under oxidative stress condition was also confirmed by the fluorogenic redox dye using flow cytometry. The results of transcriptome analysis demonstrated that the derepression of several oxidative stress genes along with the zwf-1 gene might confer high resistance to oxidative stress in the ntrC mutant. Here, we presented the data for the first time, showing that different sets of genes are involved in nitrogen-rich and nitrogen-limited oxidative stress conditions and NtrC-sensed nitrogen availability is one of the most important prerequisite for full cellular defense against oxidative stress in P. putida.",
keywords = "HexR, NADPH, Nitrogen, Soil model bacterium, SoxR, Zwf-1",
author = "Sujin Yeom and Jinki Yeom and Woojun Park",
year = "2010",
month = "4",
day = "1",
doi = "10.1007/s12275-010-0075-0",
language = "English (US)",
volume = "48",
pages = "153--159",
journal = "Journal of Microbiology",
issn = "1225-8873",
publisher = "Microbiological Society of Korea",
number = "2",

}

TY - JOUR

T1 - NtrC-sensed nitrogen availability is important for oxidative stress defense in Pseudomonas putida KT2440

AU - Yeom, Sujin

AU - Yeom, Jinki

AU - Park, Woojun

PY - 2010/4/1

Y1 - 2010/4/1

N2 - The zwf, which encodes glucose-6-phosphate dehydrogenase, is repressed by NtrC under nitrogen-limited condition. Previously, we demonstrated that induction of zwf-1 is required for protecting Pseudomonas putida cells under oxidative stress, which could be possible probably because of derepression of HexR on the zwf-1 gene under oxidative stress. These findings led us investigate that NtrC still represses the zwf-1 under nitrogen-limited oxidative stress condition, which makes cells more sensitive under such condition. Interestingly, deletion of the ntrC gene significantly reduces growth rate, but renders cells more resistant to oxidative stress, under nitrogen limited condition in P. putida. More vitality of the ntrC mutant under oxidative stress condition was also confirmed by the fluorogenic redox dye using flow cytometry. The results of transcriptome analysis demonstrated that the derepression of several oxidative stress genes along with the zwf-1 gene might confer high resistance to oxidative stress in the ntrC mutant. Here, we presented the data for the first time, showing that different sets of genes are involved in nitrogen-rich and nitrogen-limited oxidative stress conditions and NtrC-sensed nitrogen availability is one of the most important prerequisite for full cellular defense against oxidative stress in P. putida.

AB - The zwf, which encodes glucose-6-phosphate dehydrogenase, is repressed by NtrC under nitrogen-limited condition. Previously, we demonstrated that induction of zwf-1 is required for protecting Pseudomonas putida cells under oxidative stress, which could be possible probably because of derepression of HexR on the zwf-1 gene under oxidative stress. These findings led us investigate that NtrC still represses the zwf-1 under nitrogen-limited oxidative stress condition, which makes cells more sensitive under such condition. Interestingly, deletion of the ntrC gene significantly reduces growth rate, but renders cells more resistant to oxidative stress, under nitrogen limited condition in P. putida. More vitality of the ntrC mutant under oxidative stress condition was also confirmed by the fluorogenic redox dye using flow cytometry. The results of transcriptome analysis demonstrated that the derepression of several oxidative stress genes along with the zwf-1 gene might confer high resistance to oxidative stress in the ntrC mutant. Here, we presented the data for the first time, showing that different sets of genes are involved in nitrogen-rich and nitrogen-limited oxidative stress conditions and NtrC-sensed nitrogen availability is one of the most important prerequisite for full cellular defense against oxidative stress in P. putida.

KW - HexR

KW - NADPH

KW - Nitrogen

KW - Soil model bacterium

KW - SoxR

KW - Zwf-1

UR - http://www.scopus.com/inward/record.url?scp=77951988014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951988014&partnerID=8YFLogxK

U2 - 10.1007/s12275-010-0075-0

DO - 10.1007/s12275-010-0075-0

M3 - Article

C2 - 20437145

AN - SCOPUS:77951988014

VL - 48

SP - 153

EP - 159

JO - Journal of Microbiology

JF - Journal of Microbiology

SN - 1225-8873

IS - 2

ER -