NTI4F

A non-peptide fluorescent probe selective for functional delta opioid receptors

Tushar Kshirsagar, Albert H. Nakano, Ping-Yee Law, Robert Elde, Philip S Portoghese

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A non-peptide fluorescent probe, NTI4F, has been developed for the delta (δ) opioid receptor. The probe is a potent δ-antagonist in the mouse vas deferens (MVD) smooth muscle assay and it binds to the δ opioid receptor with high affinity (K(i) = 1 nM) and selectivity. Confocal microscopy indicates that the probe binds to Madin-Darby canine kidney (MDCK) cells transfected with the δ opioid receptor. This binding can be blocked by the δ opioid receptor antagonist, naltrindole (NTI), but not by morphine or ethylketazocine (EK) which are mu (μ)- and kappa (κ)-selective ligands. This fluorescent probe should prove useful in the study of the distribution of the δ opioid receptor.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalNeuroscience Letters
Volume249
Issue number2-3
DOIs
StatePublished - Jun 19 1998

Fingerprint

delta Opioid Receptor
Opioid Receptors
Fluorescent Dyes
naltrindole
Ethylketocyclazocine
Madin Darby Canine Kidney Cells
Vas Deferens
Narcotic Antagonists
Confocal Microscopy
Morphine
Smooth Muscle
Ligands
NTI4F fluorescent probe

Keywords

  • Delta opioid receptor
  • Fluorescent probe
  • Naltrindole

Cite this

NTI4F : A non-peptide fluorescent probe selective for functional delta opioid receptors. / Kshirsagar, Tushar; Nakano, Albert H.; Law, Ping-Yee; Elde, Robert; Portoghese, Philip S.

In: Neuroscience Letters, Vol. 249, No. 2-3, 19.06.1998, p. 83-86.

Research output: Contribution to journalArticle

Kshirsagar, Tushar ; Nakano, Albert H. ; Law, Ping-Yee ; Elde, Robert ; Portoghese, Philip S. / NTI4F : A non-peptide fluorescent probe selective for functional delta opioid receptors. In: Neuroscience Letters. 1998 ; Vol. 249, No. 2-3. pp. 83-86.
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